Metabolic disorders induced by high-fat feeding in rodents evoke some, if not all, of the features of human metabolic syndrome. The occurrence and severity of metabolic disorders, however, varies according to rodent species, and even strain, as well as the diet. Therefore, in the present study, we investigated the long-term obesogenic and diabetogenic effects of three high-fat diets differing by their fat/carbohydrate ratios. Sprague–Dawley rats were fed a control high-carbohydrate and low-fat diet [HCD; 3:16:6 ratio of fat/carbohydrate/protein; 15.48 kJ/g (3.7 kcal/g)], a high-fat and medium-carbohydrate diet [HFD1; 53:30:17 ratio of fat/carbohydrate/protein; 19.66 kJ/g (4.7 kcal/g)], a very-high-fat and low-carbohydrate diet [HFD2; 67:9:24 ratio of fat/carbohydrate/protein; 21.76 kJ/g (5.2 kcal/g)] or a very-high-fat and carbohydrate-free diet [HFD3; 75:0:25 ratio of fat/carbohydrate/protein; 24.69 kJ/g (5.9 kcal/g)] for 10 weeks. Compared with the control diet (HCD), rats fed with high-fat combined with more (HFD1) or less (HFD2) carbohydrate exhibited higher BMI (body mass index; +13 and +10% respectively; P<0.05) and abdominal fat (+70% in both HFD1 and HFD2; P<0.05), higher plasma leptin (+130 and +135% respectively; P<0.05), lower plasma adiponectin levels (−23 and −30% respectively; P<0.05) and impaired glucose tolerance. Only the HFD1 group had insulin resistance. By contrast, a very-high-fat diet devoid of carbohydrate (HFD3) led to impaired glucose tolerance, insulin resistance and hypoadiponectinaemia (−50%; P<0.05), whereas BMI, adiposity and plasma leptin did not differ from respective values in animals fed the control diet. We conclude that increasing the fat-to-carbohydrate ratio to the uppermost (i.e. carbohydrate-free) in a high-fat diet prevents the development of obesity, but not the prediabetic state (i.e. altered glucose tolerance and insulin sensitivity).
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November 2007
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Research Article|
October 12 2007
Increasing the fat-to-carbohydrate ratio in a high-fat diet prevents the development of obesity but not a prediabetic state in rats
Natalia Sinitskaya;
Natalia Sinitskaya
*Département de Neurobiologie des Rythmes, Institut de Neurosciences Cellulaires et Intégratives, CNRS, Université Louis Pasteur, 5 rue Blaise pascal, 67084 Strasbourg, France
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Sylviane Gourmelen;
Sylviane Gourmelen
*Département de Neurobiologie des Rythmes, Institut de Neurosciences Cellulaires et Intégratives, CNRS, Université Louis Pasteur, 5 rue Blaise pascal, 67084 Strasbourg, France
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Carole Schuster-Klein;
Carole Schuster-Klein
†Institut de Recherches Internationales Servier (IRIS-Servier), 29/31 rue du Pont, 92578 Neuilly sur Seine, France
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Béatrice Guardiola-Lemaitre;
Béatrice Guardiola-Lemaitre
†Institut de Recherches Internationales Servier (IRIS-Servier), 29/31 rue du Pont, 92578 Neuilly sur Seine, France
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Paul Pévet;
Paul Pévet
*Département de Neurobiologie des Rythmes, Institut de Neurosciences Cellulaires et Intégratives, CNRS, Université Louis Pasteur, 5 rue Blaise pascal, 67084 Strasbourg, France
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Etienne Challet
*Département de Neurobiologie des Rythmes, Institut de Neurosciences Cellulaires et Intégratives, CNRS, Université Louis Pasteur, 5 rue Blaise pascal, 67084 Strasbourg, France
Correspondence: Dr Etienne Challet (email [email protected]).
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Publisher: Portland Press Ltd
Received:
June 05 2007
Revision Received:
June 26 2007
Accepted:
July 04 2007
Accepted Manuscript online:
July 04 2007
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© The Authors Journal compilation © 2007 Biochemical Society
2007
Clin Sci (Lond) (2007) 113 (10): 417–425.
Article history
Received:
June 05 2007
Revision Received:
June 26 2007
Accepted:
July 04 2007
Accepted Manuscript online:
July 04 2007
Connected Content
This is a commentary on:
High-fat diets: healthy or unhealthy?
Citation
Natalia Sinitskaya, Sylviane Gourmelen, Carole Schuster-Klein, Béatrice Guardiola-Lemaitre, Paul Pévet, Etienne Challet; Increasing the fat-to-carbohydrate ratio in a high-fat diet prevents the development of obesity but not a prediabetic state in rats. Clin Sci (Lond) 1 November 2007; 113 (10): 417–425. doi: https://doi.org/10.1042/CS20070182
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