Diabetes mellitus accelerates atherosclerotic processes, and it is known that inflammation plays a key role in atherosclerosis. The aim of the present study was to evaluate in patients with Type 2 diabetes whether serum levels of CRP (C-reactive protein) are associated with cytokine production in whole blood. A total of 89 outpatients with Type 2 diabetes were enrolled, and blood pressure, body mass index, fasting blood glucose, glycated haemoglobin, cholesterol, triacylglycerols (triglycerides) and hs-CRP (high-sensitivity CRP) were measured. IL-6 (interleukin-6), IL-1β (interleukin-1β) and TNF-α (tumour necrosis factor-α) were measured before and after 24 h of incubation of whole blood with LPS (lipopolysaccharide) or saline. The basal values of IL-1β, IL-6 and TNF-α were low and were not significantly related to hs-CRP levels. A univariate analysis showed that the level of IL-1β and IL-6, obtained after 24 h of incubation of whole blood with LPS, increased significantly with increasing levels of hs-CRP and, after adjusting for potential confounders, IL-1β still remained statistically significant. In our sample of patients with Type 2 diabetes, there was no association between serum hs-CRP levels and basal levels of IL-6, IL-1β and TNF-α. Conversely, a significant association was observed between serum hs-CRP levels and IL-1β and IL-6 production after 24 h of incubation of whole blood with LPS. In conclusion, our data suggest that patients with Type 2 diabetes and high hs-CRP levels may have an enhanced reactivity in response to specific stimuli that produce different interleukins, with possible implications in inflammatory atherosclerotic processes.
Skip Nav Destination
Article navigation
July 2007
-
Cover Image
Cover Image
- PDF Icon PDF LinkTable of Contents
Research Article|
June 13 2007
Association between serum values of C-reactive protein and cytokine production in whole blood of patients with Type 2 diabetes Available to Purchase
Giovanna Castoldi;
*Clinica Nefrologica, Az. Ospedaliera San Gerardo, 20052 Monza, Italy
†Dipartimento di Medicina Clinica e Prevenzione, Università degli Studi di Milano-Bicocca, 20052 Monza, Italy
Correspondence: Dr Giovanna Castoldi, at the Dipartimento di Medicina Clinica e Prevenzione, Università degli Studi di Milano-Bicocca, Azienda Ospedaliera S.Gerardo di Monza, Via Pergolesi 33, 20052 Monza, Italy (email [email protected]).
Search for other works by this author on:
Stefania Galimberti;
Stefania Galimberti
†Dipartimento di Medicina Clinica e Prevenzione, Università degli Studi di Milano-Bicocca, 20052 Monza, Italy
Search for other works by this author on:
Chiara Riva;
Chiara Riva
‡Clinica Neurologica, Dipartimento di Neuroscienze, Università degli Studi di Milano-Bicocca, 20052 Monza, Italy
Search for other works by this author on:
Ruggero Papagna;
Ruggero Papagna
§Clinica Odontoiatrica, Dipartimento di Neuroscienze, Università degli Studi di Milano-Bicocca, 20052 Monza, Italy
Search for other works by this author on:
Federico Querci;
Federico Querci
∥Centro di Diabetologia, Ospedale di Alzano Lombardo, Azienda Bolognini, 24068 Seriate, Italy
Search for other works by this author on:
Marco Casati;
Marco Casati
*Clinica Nefrologica, Az. Ospedaliera San Gerardo, 20052 Monza, Italy
Search for other works by this author on:
Gianpaolo Zerbini;
Gianpaolo Zerbini
¶Unita' di Fisiopatologia Renale, Sezione Nutrizione-Metabolismo, Medicina, Istituto Scientifico San Raffaele, 20132 Milan, Italy
Search for other works by this author on:
Gianluigi Caccianiga;
Gianluigi Caccianiga
§Clinica Odontoiatrica, Dipartimento di Neuroscienze, Università degli Studi di Milano-Bicocca, 20052 Monza, Italy
Search for other works by this author on:
Carlo Ferrarese;
Carlo Ferrarese
‡Clinica Neurologica, Dipartimento di Neuroscienze, Università degli Studi di Milano-Bicocca, 20052 Monza, Italy
Search for other works by this author on:
Marco Baldoni;
Marco Baldoni
§Clinica Odontoiatrica, Dipartimento di Neuroscienze, Università degli Studi di Milano-Bicocca, 20052 Monza, Italy
Search for other works by this author on:
Maria Grazia Valsecchi;
Maria Grazia Valsecchi
†Dipartimento di Medicina Clinica e Prevenzione, Università degli Studi di Milano-Bicocca, 20052 Monza, Italy
Search for other works by this author on:
Andrea Stella
Andrea Stella
*Clinica Nefrologica, Az. Ospedaliera San Gerardo, 20052 Monza, Italy
†Dipartimento di Medicina Clinica e Prevenzione, Università degli Studi di Milano-Bicocca, 20052 Monza, Italy
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
November 29 2006
Revision Received:
March 01 2007
Accepted:
March 16 2007
Accepted Manuscript online:
March 16 2007
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© 2007 The Biochemical Society
2007
Clin Sci (Lond) (2007) 113 (2): 103–108.
Article history
Received:
November 29 2006
Revision Received:
March 01 2007
Accepted:
March 16 2007
Accepted Manuscript online:
March 16 2007
Citation
Giovanna Castoldi, Stefania Galimberti, Chiara Riva, Ruggero Papagna, Federico Querci, Marco Casati, Gianpaolo Zerbini, Gianluigi Caccianiga, Carlo Ferrarese, Marco Baldoni, Maria Grazia Valsecchi, Andrea Stella; Association between serum values of C-reactive protein and cytokine production in whole blood of patients with Type 2 diabetes. Clin Sci (Lond) 1 July 2007; 113 (2): 103–108. doi: https://doi.org/10.1042/CS20060338
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Biochemical Society Member Sign in
Sign InSign in via your Institution
Sign in via your InstitutionGet Access To This Article
Open Access for all
We offer compliant routes for all authors from 2025. With library support, there will be no author nor reader charges in 5 journals. Check here |
![]() |