The present study was designed to investigate the relationship of isoforms of adiponectin to insulin sensitivity in subjects with HIV-associated insulin resistance in response to treatment with the thiazolidinedione, rosiglitazone. The two isoforms of adiponectin, HMW (high-molecular-mass) and LMW (low-molecular-mass), were separated by sucrose-gradient-density centrifugation. The amount of adiponectin in gradient fractions was determined by ELISA. Peripheral insulin sensitivity (Rd) was determined with hyperinsulinaemic–euglycaemic clamp, whereas hepatic sensitivity [HOMA (Homoeostasis Model Assessment) %S] was based on basal glucose and insulin values. Treatment with rosiglitazone for 3 months resulted in a significant improvement in the index of hepatic insulin sensitivity (86.4±15% compared with 139±23; P=0.007) as well as peripheral insulin sensitivity (4.04±0.23 compared with 6.17±0.66 mg of glucose/kg of lean body mass per min; P<0.001). Improvement in HOMA was associated with increased levels of HMW adiponectin (r=0.541, P=0.045), but not LMW adiponectin. The present study suggests that the HMW isoform of adiponectin is important in the regulation of rosiglitazone-mediated improvement in insulin sensitivity in individuals with HIV-associated insulin resistance, particularly in the liver.
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September 2008
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Research Article|
August 12 2008
Importance of the high-molecular-mass isoform of adiponectin in improved insulin sensitivity with rosiglitazone treatment in HIV disease
Saima Qurashi;
Saima Qurashi
*Department of Medicine, Stony Brook University, Stony Brook, NY 11794, U.S.A.
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Dennis C. Mynarcik;
Dennis C. Mynarcik
*Department of Medicine, Stony Brook University, Stony Brook, NY 11794, U.S.A.
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Margaret A. McNurlan;
†Department of Surgery, Stony Brook University, Stony Brook, NY 11794, U.S.A.
Correspondence: Dr Margaret A. McNurlan (email [email protected]).
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Hongshik Ahn;
Hongshik Ahn
‡Department of Applied Mathematics and Statistics, Stony Brook University, Stony Brook, NY 11794, U.S.A.
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Robert Ferris;
Robert Ferris
*Department of Medicine, Stony Brook University, Stony Brook, NY 11794, U.S.A.
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Marie C. Gelato
Marie C. Gelato
*Department of Medicine, Stony Brook University, Stony Brook, NY 11794, U.S.A.
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Publisher: Portland Press Ltd
Received:
October 31 2007
Revision Received:
January 22 2008
Accepted:
February 05 2008
Accepted Manuscript online:
February 05 2008
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© The Authors Journal compilation © 2008 Biochemical Society
2008
Clin Sci (Lond) (2008) 115 (6): 197–202.
Article history
Received:
October 31 2007
Revision Received:
January 22 2008
Accepted:
February 05 2008
Accepted Manuscript online:
February 05 2008
Citation
Saima Qurashi, Dennis C. Mynarcik, Margaret A. McNurlan, Hongshik Ahn, Robert Ferris, Marie C. Gelato; Importance of the high-molecular-mass isoform of adiponectin in improved insulin sensitivity with rosiglitazone treatment in HIV disease. Clin Sci (Lond) 1 September 2008; 115 (6): 197–202. doi: https://doi.org/10.1042/CS20070387
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