Telomere erosion of EPCs (endothelial progenitor cells) may be a key factor in endothelial cell senescence and is highly dependent on cellular oxidative damage. The aim of the present study was to investigate whether LLT (lipid-lowering therapy) with statins could attenuate EPC telomere erosion in patients with CAD (coronary artery disease). The study included 100 patients with stable CAD and 25 subjects without CAD as controls. CAD patients were randomized to 12 months of intensive LLT with atorvastatin or moderate LLT with pravastatin. EPCs were obtained from peripheral blood at baseline and after 12 months of statin therapy. Telomere length in EPCs was measured by FISH (fluorescence in situ hybridization) and oxidative DNA damage by flow cytometry of oxidized DNA bases. EPC telomere length was shorter in the CAD group than in the controls, and oxidative DNA damage to EPCs was higher in the CAD group compared with controls. After 12 months of therapy, changes in lipid profiles were greater in the intensive LLT group than in the moderate LLT group. Intensive LLT markedly increased EPC number and decreased oxidative DNA damage in EPCs (both P<0.05), with no change in telomere length. In contrast, moderate LLT did not change EPC counts or oxidative DNA damage, but showed telomere shortening (P<0.05). There was a weak negative correlation between changes in EPC number and LDL (low-density lipoprotein)-cholesterol levels after intensive LLT, whereas there was no correlation between them after moderate LLT. With in vitro culturing of EPCs subjected to oxidative stress, atorvastatin led to the prevention of EPC telomere shortening compared with pravastatin. In conclusion, the present study has demonstrated that intensive LLT may prevent EPC telomere erosion in patients with CAD, possibly contributing to the beneficial effects of intensive LLT in this disorder.
Skip Nav Destination
Article navigation
June 2009
-
Cover Image
Cover Image
- PDF Icon PDF LinkTable of Contents
Research Article|
May 01 2009
Effect of intensive lipid-lowering therapy on telomere erosion in endothelial progenitor cells obtained from patients with coronary artery disease
Mamoru Satoh;
1Division of Cardiology and Memorial Heart Center, Department of Internal Medicine, Iwate Medical University School of Medicine, Morioka 020-8505, Iwate, Japan
Correspondence: Dr Mamoru Satoh (email [email protected]).
Search for other works by this author on:
Yoshitaka Minami;
Yoshitaka Minami
1Division of Cardiology and Memorial Heart Center, Department of Internal Medicine, Iwate Medical University School of Medicine, Morioka 020-8505, Iwate, Japan
Search for other works by this author on:
Yuji Takahashi;
Yuji Takahashi
1Division of Cardiology and Memorial Heart Center, Department of Internal Medicine, Iwate Medical University School of Medicine, Morioka 020-8505, Iwate, Japan
Search for other works by this author on:
Tsuyoshi Tabuchi;
Tsuyoshi Tabuchi
1Division of Cardiology and Memorial Heart Center, Department of Internal Medicine, Iwate Medical University School of Medicine, Morioka 020-8505, Iwate, Japan
Search for other works by this author on:
Tomonori Itoh;
Tomonori Itoh
1Division of Cardiology and Memorial Heart Center, Department of Internal Medicine, Iwate Medical University School of Medicine, Morioka 020-8505, Iwate, Japan
Search for other works by this author on:
Motoyuki Nakamura
Motoyuki Nakamura
1Division of Cardiology and Memorial Heart Center, Department of Internal Medicine, Iwate Medical University School of Medicine, Morioka 020-8505, Iwate, Japan
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
August 14 2008
Revision Received:
December 15 2008
Accepted:
December 17 2008
Accepted Manuscript online:
December 17 2008
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© The Authors Journal compilation © 2009 Biochemical Society
2009
Clin Sci (Lond) (2009) 116 (11): 827–835.
Article history
Received:
August 14 2008
Revision Received:
December 15 2008
Accepted:
December 17 2008
Accepted Manuscript online:
December 17 2008
Citation
Mamoru Satoh, Yoshitaka Minami, Yuji Takahashi, Tsuyoshi Tabuchi, Tomonori Itoh, Motoyuki Nakamura; Effect of intensive lipid-lowering therapy on telomere erosion in endothelial progenitor cells obtained from patients with coronary artery disease. Clin Sci (Lond) 1 June 2009; 116 (11): 827–835. doi: https://doi.org/10.1042/CS20080404
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Biochemical Society Member Sign in
Sign InSign in via your Institution
Sign in via your InstitutionGet Access To This Article
Open Access for all
We offer compliant routes for all authors from 2025. With library support, there will be no author nor reader charges in 5 journals. Check here |
![]() |