The transition from the fed to the fasted resting state is characterized by, among other things, changes in lipid metabolism and peripheral insulin resistance. Acylcarnitines have been suggested to play a role in insulin resistance, as well as other long-chain fatty acid metabolites. Plasma levels of long-chain acylcarnitines increase during fasting, but this is unknown for muscle long-chain acylcarnitines. In the present study we investigated whether muscle long-chain acylcarnitines increase during fasting and we investigated their relationship with glucose/fat oxidation and insulin sensitivity in lean healthy humans. After 14 h and 62 h of fasting, glucose fluxes, substrate oxidation, and plasma and muscle acylcarnitines were measured before and during a hyperinsulinaemic–euglycaemic clamp. Hyperinsulinaemia decreased long-chain muscle acylcarnitines after 14 h of fasting, but not after 62 h of fasting. In both the basal state and during the clamp, glucose oxidation was lower and fatty acid oxidation was higher after 62 h compared with 14 h of fasting. Absolute changes in glucose and fatty acid oxidation in the basal compared with hyperinsulinaemic state were not different. Muscle long-chain acylcarnitines did not correlate with glucose oxidation, fatty acid oxidation or insulin-mediated peripheral glucose uptake. After 62 h of fasting, the suppression of muscle long-chain acylcarnitines by insulin was attenuated compared with 14 h of fasting. Muscle long-chain acylcarnitines do not unconditionally reflect fatty acid oxidation. The higher fatty acid oxidation during hyperinsulinaemia after 62 h compared with 14 h of fasting, although the absolute decrease in fatty acid oxidation was not different, suggests a different set point.
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April 2009
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Research Article|
March 02 2009
Muscle acylcarnitines during short-term fasting in lean healthy men
Maarten R. Soeters;
*Department of Endocrinology and Metabolism, Academic Medical Center, University of Amsterdam, Amsterdam 1105 AZ, The Netherlands
Correspondence: Dr M. R. Soeters (email [email protected]).
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Hans P. Sauerwein;
Hans P. Sauerwein
*Department of Endocrinology and Metabolism, Academic Medical Center, University of Amsterdam, Amsterdam 1105 AZ, The Netherlands
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Marinus Duran;
Marinus Duran
†Department of Clinical Chemistry, Laboratory Genetic Metabolic Diseases, Academic Medical Center, University of Amsterdam, Amsterdam 1105 AZ, The Netherlands
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Ronald J. Wanders;
Ronald J. Wanders
†Department of Clinical Chemistry, Laboratory Genetic Metabolic Diseases, Academic Medical Center, University of Amsterdam, Amsterdam 1105 AZ, The Netherlands
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Mariëtte T. Ackermans;
Mariëtte T. Ackermans
‡Department of Clinical Chemistry, Laboratory of Endocrinology, Academic Medical Center, University of Amsterdam, Amsterdam 1105 AZ, The Netherlands
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Eric Fliers;
Eric Fliers
*Department of Endocrinology and Metabolism, Academic Medical Center, University of Amsterdam, Amsterdam 1105 AZ, The Netherlands
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Sander M. Houten;
Sander M. Houten
†Department of Clinical Chemistry, Laboratory Genetic Metabolic Diseases, Academic Medical Center, University of Amsterdam, Amsterdam 1105 AZ, The Netherlands
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Mireille J. Serlie
Mireille J. Serlie
*Department of Endocrinology and Metabolism, Academic Medical Center, University of Amsterdam, Amsterdam 1105 AZ, The Netherlands
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Publisher: Portland Press Ltd
Received:
August 27 2008
Revision Received:
October 20 2008
Accepted:
October 22 2008
Accepted Manuscript online:
October 22 2008
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© The Authors Journal compilation © 2009 Biochemical Society
2009
Clin Sci (Lond) (2009) 116 (7): 585–592.
Article history
Received:
August 27 2008
Revision Received:
October 20 2008
Accepted:
October 22 2008
Accepted Manuscript online:
October 22 2008
Citation
Maarten R. Soeters, Hans P. Sauerwein, Marinus Duran, Ronald J. Wanders, Mariëtte T. Ackermans, Eric Fliers, Sander M. Houten, Mireille J. Serlie; Muscle acylcarnitines during short-term fasting in lean healthy men. Clin Sci (Lond) 1 April 2009; 116 (7): 585–592. doi: https://doi.org/10.1042/CS20080433
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