In the present study, we have analysed both the effect of long-term portal hypertension on the vasomotor response to acetylcholine in rat aorta and the mechanism involved in this response. For this purpose, sham-operated rats and rats with pre-hepatic PH (portal hypertension; triple partial portal vein ligation) were used at 21 months after surgery. The participation of NO and COX (cyclo-oxygenase) derivatives in the vasodilator response elicited by acetylcholine after incubation with L-NAME (NG-nitro-L-arginine methyl ester), indomethacin, SC-560, NS-398, tranylcypromine and furegrelate, was analysed. NO, TXB2 (thromboxane B2) and 6-keto PGF1α (prostaglandin F1α) release were measured. In addition, SNP (sodium nitroprusside), U-46619, PGI2 and forskolin vasomotor responses were analysed. COX-1 and COX-2 expression was also determined. The acetylcholine-induced vasodilating response was higher in rats with PH. TXA2 and NO release, and SNP and U-46619 sensitivity were similar in both groups. PGI2 release was not modified by portal hypertension, but vasodilator responses to this prostanoid and to forskolin were higher in rats with PH. COX-1 and COX-2 expression remained unmodified by surgery. In conclusion, increased vasodilation to acetylcholine is maintained in long-term PH. Although the participation of endothelial NO remained unmodified, the COX-2 derivative PGI2 does participate through an increased vasodilator response.
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Research Article|
September 07 2009
Long-term portal hypertension increases the vasodilator response to acetylcholine in rat aorta: role of prostaglandin I2
Javier Blanco-Rivero;
Javier Blanco-Rivero
*Departamento de Fisiología, Facultad de Medicina, Universidad Autónoma de Madrid, 28029 Madrid, Spain
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Ma Angeles Aller;
Ma Angeles Aller
†Departamento de Cirugía I, Facultad de Medicina, Universidad Complutense de Madrid, 28040 Madrid, Spain
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Jaime Arias;
Jaime Arias
†Departamento de Cirugía I, Facultad de Medicina, Universidad Complutense de Madrid, 28040 Madrid, Spain
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Mercedes Ferrer;
Mercedes Ferrer
*Departamento de Fisiología, Facultad de Medicina, Universidad Autónoma de Madrid, 28029 Madrid, Spain
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Gloria Balfagón
*Departamento de Fisiología, Facultad de Medicina, Universidad Autónoma de Madrid, 28029 Madrid, Spain
Correspondence: Dr Gloria Balfagón (email [email protected]).
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Publisher: Portland Press Ltd
Received:
September 30 2008
Revision Received:
February 03 2009
Accepted:
March 30 2009
Accepted Manuscript online:
March 30 2009
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© The Authors Journal compilation © 2009 Biochemical Society
2009
Clin Sci (Lond) (2009) 117 (10): 365–374.
Article history
Received:
September 30 2008
Revision Received:
February 03 2009
Accepted:
March 30 2009
Accepted Manuscript online:
March 30 2009
Citation
Javier Blanco-Rivero, Ma Angeles Aller, Jaime Arias, Mercedes Ferrer, Gloria Balfagón; Long-term portal hypertension increases the vasodilator response to acetylcholine in rat aorta: role of prostaglandin I2. Clin Sci (Lond) 1 November 2009; 117 (10): 365–374. doi: https://doi.org/10.1042/CS20080499
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