ODM (offspring of diabetic mothers) have an increased risk of developing metabolic and cardiovascular dysfunction; however, few studies have focused on the susceptibility to disease in offspring of mothers developing diabetes during pregnancy. We developed an animal model of late gestation diabetic pregnancy and characterized metabolic and vascular function in the offspring. Diabetes was induced by streptozotocin (50 mg/kg of body weight, intraperitoneally) in pregnant rats on gestational day 13 and was partially controlled by twice-daily injections of insulin. At 2 months of age, ODM had slightly better glucose tolerance than controls (P<0.05); however, by 6 months of age this trend had reversed. A euglycaemic–hyperinsulinamic clamp revealed insulin resistance in male ODM (P<0.05). In 6–8-month-old female ODM, aortas had significantly enhanced contractility in response to KCl, ET-1 (endothelin-1) and NA (noradrenaline). No differences in responses to ET-1 and NA were apparent with co-administration of L-NNA (NG-nitro-L-arginine). Relaxation in response to ACh (acetylcholine), but not SNP (sodium nitroprusside), was significantly impaired in female ODM. In contrast, males had no between-group differences in response to vasoconstrictors, whereas relaxation to SNP and ACh was greater in ODM compared with control animals. Thus the development of diabetes during pregnancy programmes gender-specific insulin resistance and vascular dysfunction in adult offspring.
Skip Nav Destination
Article navigation
August 2009
-
Cover Image
Cover Image
- PDF Icon PDF LinkTable of Contents
Research Article|
July 02 2009
Programming of growth, insulin resistance and vascular dysfunction in offspring of late gestation diabetic rats
Emily M. Segar;
Emily M. Segar
1Department of Pediatrics, University of Iowa Carver College of Medicine, Iowa City, IA 52242, U.S.A.
Search for other works by this author on:
Andrew W. Norris;
Andrew W. Norris
1Department of Pediatrics, University of Iowa Carver College of Medicine, Iowa City, IA 52242, U.S.A.
Search for other works by this author on:
Jian-Rong Yao;
Jian-Rong Yao
1Department of Pediatrics, University of Iowa Carver College of Medicine, Iowa City, IA 52242, U.S.A.
Search for other works by this author on:
Shanming Hu;
Shanming Hu
1Department of Pediatrics, University of Iowa Carver College of Medicine, Iowa City, IA 52242, U.S.A.
Search for other works by this author on:
Stacia L. Koppenhafer;
Stacia L. Koppenhafer
1Department of Pediatrics, University of Iowa Carver College of Medicine, Iowa City, IA 52242, U.S.A.
Search for other works by this author on:
Robert D. Roghair;
Robert D. Roghair
1Department of Pediatrics, University of Iowa Carver College of Medicine, Iowa City, IA 52242, U.S.A.
Search for other works by this author on:
Jeffrey L. Segar;
1Department of Pediatrics, University of Iowa Carver College of Medicine, Iowa City, IA 52242, U.S.A.
Correspondence: Professor Jeffrey L. Segar (email [email protected]).
Search for other works by this author on:
Thomas D. Scholz
Thomas D. Scholz
1Department of Pediatrics, University of Iowa Carver College of Medicine, Iowa City, IA 52242, U.S.A.
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
October 22 2008
Revision Received:
January 30 2009
Accepted:
February 09 2009
Accepted Manuscript online:
February 09 2009
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© The Authors Journal compilation © 2009 Biochemical Society
2009
Clin Sci (Lond) (2009) 117 (3): 129–138.
Article history
Received:
October 22 2008
Revision Received:
January 30 2009
Accepted:
February 09 2009
Accepted Manuscript online:
February 09 2009
Citation
Emily M. Segar, Andrew W. Norris, Jian-Rong Yao, Shanming Hu, Stacia L. Koppenhafer, Robert D. Roghair, Jeffrey L. Segar, Thomas D. Scholz; Programming of growth, insulin resistance and vascular dysfunction in offspring of late gestation diabetic rats. Clin Sci (Lond) 1 August 2009; 117 (3): 129–138. doi: https://doi.org/10.1042/CS20080550
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Biochemical Society Member Sign in
Sign InSign in via your Institution
Sign in via your InstitutionGet Access To This Article
Open Access for all
We offer compliant routes for all authors from 2025. With library support, there will be no author nor reader charges in 5 journals. Check here |
![]() |