SCFAs (short-chain fatty acids) are produced by anaerobic bacterial fermentation. Increased concentrations of these fatty acids are observed in inflammatory conditions, such as periodontal disease, and at sites of anaerobic infection. In the present study, the effect of the SCFAs acetate, propionate and butyrate on neutrophil chemotaxis and migration was investigated. Experiments were carried out in rats and in vitro. The following parameters were measured: rolling, adherence, expression of adhesion molecules in neutrophils (L-selectin and β2 integrin), transmigration, air pouch influx of neutrophils and production of cytokines [CINC-2αβ (cytokine-induced neutrophil chemoattractant-2αβ), IL-1β (interleukin-1β), MIP-1α (macrophage inflammatory protein-1α) and TNF-α (tumour necrosis factor-α)]. SCFAs induced in vivo neutrophil migration and increased the release of CINC-2αβ into the air pouch. These fatty acids increased the number of rolling and adhered cells as evaluated by intravital microscopy. SCFA treatment increased L-selectin expression on the neutrophil surface and L-selectin mRNA levels, but had no effect on the expression of β2 integrin. Propionate and butyrate also increased in vitro transmigration of neutrophils. These results indicate that SCFAs produced by anaerobic bacteria raise neutrophil migration through increased L-selectin expression on neutrophils and CINC-2αβ release.
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Research Article|
September 01 2009
Short-chain fatty acids stimulate the migration of neutrophils to inflammatory sites
Marco A. R. Vinolo;
*Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo, 05508-900, Butantã, Sao Paulo, Brazil
Correspondence: Mr Marco A.R. Vinolo (email [email protected]).
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Hosana G. Rodrigues;
Hosana G. Rodrigues
*Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo, 05508-900, Butantã, Sao Paulo, Brazil
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Elaine Hatanaka;
Elaine Hatanaka
*Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo, 05508-900, Butantã, Sao Paulo, Brazil
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Cristina B. Hebeda;
Cristina B. Hebeda
†Department of Clinical and Toxicological Analyses, Faculty of Pharmaceutical Sciences, University of Sao Paulo, 05508-900, Butantã, Sao Paulo, Brazil
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Sandra H. P. Farsky;
Sandra H. P. Farsky
†Department of Clinical and Toxicological Analyses, Faculty of Pharmaceutical Sciences, University of Sao Paulo, 05508-900, Butantã, Sao Paulo, Brazil
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Rui Curi
Rui Curi
*Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo, 05508-900, Butantã, Sao Paulo, Brazil
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Publisher: Portland Press Ltd
Received:
December 09 2008
Revision Received:
March 16 2009
Accepted:
April 01 2009
Accepted Manuscript online:
April 01 2009
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© The Authors Journal compilation © 2009 Biochemical Society
2009
Clin Sci (Lond) (2009) 117 (9): 331–338.
Article history
Received:
December 09 2008
Revision Received:
March 16 2009
Accepted:
April 01 2009
Accepted Manuscript online:
April 01 2009
Citation
Marco A. R. Vinolo, Hosana G. Rodrigues, Elaine Hatanaka, Cristina B. Hebeda, Sandra H. P. Farsky, Rui Curi; Short-chain fatty acids stimulate the migration of neutrophils to inflammatory sites. Clin Sci (Lond) 1 November 2009; 117 (9): 331–338. doi: https://doi.org/10.1042/CS20080642
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