AngII (angiotensin II), ACE (angiotensin I-converting enzyme) and the AT1 receptor (AngII type 1 receptor) are associated with the inflammatory process and microvascular dysfunction of AKI (acute kidney injury) induced by renal I/R (ischaemia/reperfusion). However, Ang-(1–7) [angiotensin-(1–7)], ACE2 (angiotensin I-converting enzyme 2) and the Mas receptor also play a role in renal disease models. Therefore, in the present study, we have examined the renal profile of Ang-(1–7), ACE2 and the Mas receptor in renal I/R and compared them with that of AngII, ACE and the AT1 receptor. Male Wistar rats were submitted to left nephrectomy and ischaemia (45 min) followed by reperfusion (2 or 4 h) in the right kidney. At 4 h of reperfusion, renal AngII was increased (P<0.01) and renal Ang-(1–7) was decreased substantially (P<0.05), although plasma levels of both angiotensins were unchanged. In addition, renal I/R decreased the renal mRNA expression of renin (P<0.05), AT1 receptors (P<0.001) and ACE2 (P<0.05). At 2 and 4 h of reperfusion, renal ACE activity was reduced (P<0.05). On the other hand, renal expression of the Mas receptor was greatly increased at 4 h of reperfusion (P<0.01), which was confirmed by immunohistochemical and Western blot analysis. In conclusion, increased renal expression of the Mas receptor associated with changes in the RAS (renin–angiotensin system)-related peptidases support an important role for the ACE2–Ang-(1–7)–Mas axis in AKI.
ACE2–angiotensin-(1–7)–Mas axis in renal ischaemia/reperfusion injury in rats
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Kátia D. da Silveira, Kênia S. Pompermayer Bosco, Lúcio R. L. Diniz, Adriana K. Carmona, Giovanni D. Cassali, Oscar Bruna-Romero, Lirlândia P. de Sousa, Mauro M. Teixeira, Robson A. S. Santos, Ana C. Simões e Silva, Maria A. Ribeiro Vieira; ACE2–angiotensin-(1–7)–Mas axis in renal ischaemia/reperfusion injury in rats. Clin Sci (Lond) 1 October 2010; 119 (9): 385–394. doi: https://doi.org/10.1042/CS20090554
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