The aim of the present study was to examine the effects of obesity alone and obesity associated with Type 2 diabetes on the structure, vascular reactivity and response to insulin of isolated human subcutaneous fat arterioles; these effects were correlated with the expression of insulin signalling proteins. Periumbilical subcutaneous adipose tissue was explanted during surgery, small arterioles (internal diameter 220±40 μm) were dissected out and investigated by electron microscopy, myography and immunoblotting. Compared with the subcutaneous arterioles of lean subjects, obesity activated the endothelium, enhanced the accumulation of collagen within vascular wall and increased the sensitivity of adrenergic response; obesity also diminished eNOS (endothelial NO synthase) protein expression, NO production, and endothelium-dependent and insulin-induced vasodilatation, as well as the protein expression of both IRS (insulin receptor substrates)-1 and IRS-2 and of the downstream molecules in the insulin signalling pathway, such as PI3K (phosphoinositide 3-kinase), phospho-Akt and Akt. When obesity was associated with Type 2 diabetes, these changes were significantly augmented. In conclusion, obesity alone or obesity associated with Type 2 diabetes alters human periumbilical adipose tissue arterioles in terms of structure, function and biochemsitry, including diminished eNOS expression and reduced levels of IRS-1, IRS-2, PI3K and Akt in the insulin signalling pathway.
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Research Article|
January 28 2011
Dysfunction of human subcutaneous fat arterioles in obesity alone or obesity associated with Type 2 diabetes
Adriana Georgescu;
*‘Petru Poni’ Institute of Macromolecular Chemistry, Iasi, Romania
†Institute of Cellular Biology and Pathology ‘Nicolae Simionescu’, Bucharest, Romania
Correspondence: Dr Adriana Georgescu (email [email protected]).
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Doina Popov;
Doina Popov
†Institute of Cellular Biology and Pathology ‘Nicolae Simionescu’, Bucharest, Romania
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Anamaria Constantin;
Anamaria Constantin
†Institute of Cellular Biology and Pathology ‘Nicolae Simionescu’, Bucharest, Romania
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Miruna Nemecz;
Miruna Nemecz
†Institute of Cellular Biology and Pathology ‘Nicolae Simionescu’, Bucharest, Romania
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Nicoleta Alexandru;
Nicoleta Alexandru
*‘Petru Poni’ Institute of Macromolecular Chemistry, Iasi, Romania
†Institute of Cellular Biology and Pathology ‘Nicolae Simionescu’, Bucharest, Romania
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Daniel Cochior;
Daniel Cochior
‡CFR 2 University Hospital, Bucharest, Romania
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Aura Tudor
Aura Tudor
‡CFR 2 University Hospital, Bucharest, Romania
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Publisher: Portland Press Ltd
Received:
July 08 2010
Revision Received:
October 14 2010
Accepted:
October 28 2010
Accepted Manuscript online:
October 28 2010
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© The Authors Journal compilation © 2011 Biochemical Society
2011
Clin Sci (Lond) (2011) 120 (10): 463–472.
Article history
Received:
July 08 2010
Revision Received:
October 14 2010
Accepted:
October 28 2010
Accepted Manuscript online:
October 28 2010
Citation
Adriana Georgescu, Doina Popov, Anamaria Constantin, Miruna Nemecz, Nicoleta Alexandru, Daniel Cochior, Aura Tudor; Dysfunction of human subcutaneous fat arterioles in obesity alone or obesity associated with Type 2 diabetes. Clin Sci (Lond) 1 May 2011; 120 (10): 463–472. doi: https://doi.org/10.1042/CS20100355
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