Resistin may play a major potential role in vascular remodelling and may contribute to atherogenesis. However, the role of VSMC (vascular smooth muscle cell)-derived resistin in neointimal formation is not well understood. We hypothesize that endogenous resistin derived from VSMCs may contribute to neointimal formation after vascular injury. VSMCs from thoracic aorta of adult Wistar rats were cultured. The carotid artery from adult Wistar rats was injured by balloon catheter. Resistin significantly increased migration and proliferation of VSMCs. Resistin siRNA (small interfering RNA) and resistin antibody significantly inhibited migration and proliferation of VSMCs induced by conditioned medium from stretched VSMCs. Resistin protein and mRNA expression significantly increased at 14 days after carotid injury. Resistin siRNA and NAC (N-acetylcysteine) significantly reduced resistin protein and mRNA expression induced by balloon injury. Carotid artery injury increased ROS (reactive oxygen species) production. Treatment with NAC and resistin siRNA decreased ROS production. The neointimal area was significantly increased after carotid injury and was significantly reduced by resistin siRNA and NAC. In conclusion, resistin increases migration and proliferation of VSMCs, and expression of resistin in carotid artery significantly increases after injury. Resistin siRNA attenuates neointimal formation after carotid injury partly through an antioxidative mechanism. Resistin may play a pivotal role in the pathogenesis of neointimal thickening after mechanical injury.
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Research Article|
October 20 2010
Resistin contributes to neointimal formation via oxidative stress after vascular injury
Kou-Gi Shyu;
Kou-Gi Shyu
*Division of Cardiology, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan
†Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan
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Li-Ming Lien;
Li-Ming Lien
‡Department of Neurology, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan
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Bao-Wei Wang;
Bao-Wei Wang
*Division of Cardiology, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan
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Peiliang Kuan;
Peiliang Kuan
*Division of Cardiology, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan
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Hang Chang
§Department of Emergency Medicine, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan
Correspondence: Professor Hang Chang (email [email protected]).
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Publisher: Portland Press Ltd
Received:
April 23 2010
Revision Received:
August 25 2010
Accepted:
August 26 2010
Accepted Manuscript online:
August 26 2010
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© The Authors Journal compilation © 2011 Biochemical Society
2011
Clin Sci (Lond) (2011) 120 (3): 121–129.
Article history
Received:
April 23 2010
Revision Received:
August 25 2010
Accepted:
August 26 2010
Accepted Manuscript online:
August 26 2010
Connected Content
A retraction has been published:
Retraction: Resistin contributes to neointimal formation via oxidative stress after vascular injury
Citation
Kou-Gi Shyu, Li-Ming Lien, Bao-Wei Wang, Peiliang Kuan, Hang Chang; Resistin contributes to neointimal formation via oxidative stress after vascular injury. Clin Sci (Lond) 1 February 2011; 120 (3): 121–129. doi: https://doi.org/10.1042/CS20100226
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