HCV (hepatitis C virus) infection is a serious health care problem that affects more than 170 million people worldwide. Viral clearance depends on the development of a successful cellular immune response against the virus. Interestingly, such a response is altered in chronically infected patients, leading to chronic hepatitis that can result in liver fibrosis, cirrhosis and hepatocellular carcinoma. Among the mechanisms that have been described as being responsible for the immune suppression caused by the virus, Treg-cells (regulatory T-cells) are emerging as an essential component. In the present work we aim to study the effect of HCV-core protein in the development of T-cells with regulatory-like function. Using a third-generation lentiviral system to express HCV-core in CD4+ Jurkat T-cells, we describe that HCV-core-expressing Jurkat cells show an up-regulation of FOXP3 (forkhead box P3) and CTLA-4 (cytotoxic T-lymphocyte antigen-4). Moreover, we show that HCV-core-transduced Jurkat cells are able to suppress CD4+ and CD8+ T-cell responses to anti-CD3 plus anti-CD28 stimulation.
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Research Article|
March 12 2012
Up-regulation of FOXP3 and induction of suppressive function in CD4+ Jurkat T-cells expressing hepatitis C virus core protein
Margarita Dominguez-Villar;
Margarita Dominguez-Villar
1Puerto Real University Hospital Research Unit, School of Medicine, Department of Biomedicine, Biotechnology (Immunology), University of Cadiz, Cadiz, Spain
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Cecilia Fernandez-Ponce;
Cecilia Fernandez-Ponce
1Puerto Real University Hospital Research Unit, School of Medicine, Department of Biomedicine, Biotechnology (Immunology), University of Cadiz, Cadiz, Spain
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Alba Munoz-Suano;
Alba Munoz-Suano
1Puerto Real University Hospital Research Unit, School of Medicine, Department of Biomedicine, Biotechnology (Immunology), University of Cadiz, Cadiz, Spain
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Esperanza Gomez;
Esperanza Gomez
1Puerto Real University Hospital Research Unit, School of Medicine, Department of Biomedicine, Biotechnology (Immunology), University of Cadiz, Cadiz, Spain
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Manuel Rodríguez-Iglesias;
Manuel Rodríguez-Iglesias
1Puerto Real University Hospital Research Unit, School of Medicine, Department of Biomedicine, Biotechnology (Immunology), University of Cadiz, Cadiz, Spain
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Francisco Garcia-Cozar
1Puerto Real University Hospital Research Unit, School of Medicine, Department of Biomedicine, Biotechnology (Immunology), University of Cadiz, Cadiz, Spain
Correspondence: Associate Professor Francisco Garcia-Cozar (email [email protected]).
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Publisher: Portland Press Ltd
Received:
December 05 2011
Revision Received:
December 23 2011
Accepted:
January 03 2012
Accepted Manuscript online:
January 03 2012
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© The Authors Journal compilation © 2012 Biochemical Society
2012
Clin Sci (Lond) (2012) 123 (1): 15–27.
Article history
Received:
December 05 2011
Revision Received:
December 23 2011
Accepted:
January 03 2012
Accepted Manuscript online:
January 03 2012
Citation
Margarita Dominguez-Villar, Cecilia Fernandez-Ponce, Alba Munoz-Suano, Esperanza Gomez, Manuel Rodríguez-Iglesias, Francisco Garcia-Cozar; Up-regulation of FOXP3 and induction of suppressive function in CD4+ Jurkat T-cells expressing hepatitis C virus core protein. Clin Sci (Lond) 1 July 2012; 123 (1): 15–27. doi: https://doi.org/10.1042/CS20110631
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