A previous expression profiling of VAT (visceral adipose tissue) revealed that the TSLP (thymic stromal lymphopoietin) gene was less expressed in severely obese men with (n=7) compared with without (n=7) the MetS (metabolic syndrome). We hypothesized that TSLP SNPs (single nucleotide polymorphisms) are associated with TSLP gene expression in VAT and with MetS phenotypes. Following validation of lower TSLP expression (P=0.003) in VAT of severely obese men and women with (n=70) compared with without (n=60) the MetS, a detailed genetic investigation was performed at the TSLP locus by sequencing its promoter, exons and intron–exon splicing boundaries using DNA of 25 severely obese subjects. Five tagging SNPs were genotyped in the 130 subjects from the expression analysis to test whether these SNPs contributed to TSLP expression variability (ANOVAs) and then genotyped in two independent samples of severely obese men (total, n=389) and women (total, n=894). In a sex-stratified multistage experimental design, ANOVAs were performed to test whether tagging SNPs were associated with MetS components treated as continuous variables. We observed that the non-coding SNP rs2289277 was associated with TSLP mRNA abundance (P=0.04), as well as with SBP [systolic BP (blood pressure)] (P=0.004) and DBP (diastolic BP) (P=0.0003) in men when adjusting for age, waist circumference, smoking and medication treating hypertension. These novel observations suggest that TSLP expression in VAT may partly explain the inter-individual variability for metabolic impairments in the presence of obesity and that specific SNPs (rs2289277 and/or correlating SNPs) may influence TSLP gene expression as well as BP in obese men.
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July 2012
Research Article|
March 27 2012
Thymic stromal lymphopoietin: an immune cytokine gene associated with the metabolic syndrome and blood pressure in severe obesity
Valérie Turcot;
Valérie Turcot
*Institute of Nutraceuticals and Functional Foods, 2440 Hochelaga Blvd., Quebec City, Canada G1V 0A6
†Molecular Endocrinology and Genomics, CHUL Research Center, 2705 Laurier Blvd., Quebec City, Canada G1V 4G2
‡Department of Food Sciences and Nutrition, Université Laval, 2425 de l'Agriculture Av., Quebec City, Canada G1V 0A6
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Luigi Bouchard;
Luigi Bouchard
§Department of Biochemistry, Université de Sherbrooke, and ECOGENE-21 Research Center, Chicoutimi Hospital, 305 Saint-Vallier St., Chicoutimi, Canada G7H 5H6
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Geneviève Faucher;
Geneviève Faucher
*Institute of Nutraceuticals and Functional Foods, 2440 Hochelaga Blvd., Quebec City, Canada G1V 0A6
‡Department of Food Sciences and Nutrition, Université Laval, 2425 de l'Agriculture Av., Quebec City, Canada G1V 0A6
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Véronique Garneau;
Véronique Garneau
*Institute of Nutraceuticals and Functional Foods, 2440 Hochelaga Blvd., Quebec City, Canada G1V 0A6
‡Department of Food Sciences and Nutrition, Université Laval, 2425 de l'Agriculture Av., Quebec City, Canada G1V 0A6
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André Tchernof;
André Tchernof
†Molecular Endocrinology and Genomics, CHUL Research Center, 2705 Laurier Blvd., Quebec City, Canada G1V 4G2
‡Department of Food Sciences and Nutrition, Université Laval, 2425 de l'Agriculture Av., Quebec City, Canada G1V 0A6
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Yves Deshaies;
Yves Deshaies
‖Department of Medicine, Université Laval, 1050 de la Médecine Av., Quebec City, Canada G1V 0A6
¶Quebec Heart and Lung Institute, 2725 Sainte-Foy Rd., Quebec City, Canada G1V 4G5
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Louis Pérusse;
Louis Pérusse
**Department of Preventive Medicine, Université Laval, 1050 de la Médecine Av., Quebec City, Canada G1V 0A6
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Simon Marceau;
Simon Marceau
¶Quebec Heart and Lung Institute, 2725 Sainte-Foy Rd., Quebec City, Canada G1V 4G5
††Department of Surgery, Université Laval, 1050 de la Médecine Av., Quebec City, Canada G1V 0A6
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Simon Biron;
Simon Biron
¶Quebec Heart and Lung Institute, 2725 Sainte-Foy Rd., Quebec City, Canada G1V 4G5
††Department of Surgery, Université Laval, 1050 de la Médecine Av., Quebec City, Canada G1V 0A6
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Odette Lescelleur;
Odette Lescelleur
¶Quebec Heart and Lung Institute, 2725 Sainte-Foy Rd., Quebec City, Canada G1V 4G5
††Department of Surgery, Université Laval, 1050 de la Médecine Av., Quebec City, Canada G1V 0A6
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Laurent Biertho;
Laurent Biertho
¶Quebec Heart and Lung Institute, 2725 Sainte-Foy Rd., Quebec City, Canada G1V 4G5
††Department of Surgery, Université Laval, 1050 de la Médecine Av., Quebec City, Canada G1V 0A6
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Marie-Claude Vohl
*Institute of Nutraceuticals and Functional Foods, 2440 Hochelaga Blvd., Quebec City, Canada G1V 0A6
†Molecular Endocrinology and Genomics, CHUL Research Center, 2705 Laurier Blvd., Quebec City, Canada G1V 4G2
‡Department of Food Sciences and Nutrition, Université Laval, 2425 de l'Agriculture Av., Quebec City, Canada G1V 0A6
Correspondence: Dr Marie-Claude Vohl (email marie-claude.vohl@fsaa.ulaval.ca).
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Clin Sci (Lond) (2012) 123 (2): 99–109.
Article history
Received:
November 16 2011
Revision Received:
January 03 2012
Accepted:
February 03 2012
Accepted Manuscript online:
February 03 2012
Connected Content
A correction has been published:
Thymic stromal lymphopoietin: an immune cytokine gene associated with the metabolic syndrome and blood pressure in severe obesity
Citation
Valérie Turcot, Luigi Bouchard, Geneviève Faucher, Véronique Garneau, André Tchernof, Yves Deshaies, Louis Pérusse, Simon Marceau, Simon Biron, Odette Lescelleur, Laurent Biertho, Marie-Claude Vohl; Thymic stromal lymphopoietin: an immune cytokine gene associated with the metabolic syndrome and blood pressure in severe obesity. Clin Sci (Lond) 1 July 2012; 123 (2): 99–109. doi: https://doi.org/10.1042/CS20110584
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