DC (dendritic cells) play an important role in the immune system. They invade peripheral tissues to detect harmful antigens, inducing a local immune response. Studies suggest that DCPs (dendritic cell precursors) might be reduced in AMI (acute myocardial infarction); however, the reason for their reduction is unknown yet. In the present study, circulating mDCPs (myeloid DCPs), pDCPs (plasmacytoid DCPs), tDCPs (total DCPs) and serum levels of TNFα (tumour necrosis factor α), IL (interleukin)-2, -4, -5, -6, -10 and -12 were analysed by flow cytometry in blood of patients with NSTEMI [non-STEMI (ST-segment elevation myocardial infarction)] (n=44) and STEMI (n=34) compared with controls with excluded CAD (coronary artery disease) (n=45). Post-mortem myocardial specimens of patients with AMI (n=12) and healthy myocardium of accident victims (n=10) were immunostained for mDCs (myeloid dendritic cells) T-cells and macrophages. Compared with controls, in patients with AMI a significant decrease in circulating mDCPs, pDCPs and tDCPs was observed (each P<0.0001). The extent of the decrease was higher in STEMI than NSTEMI patients. Serum levels were significantly higher in patients with AMI compared with controls for IL-6, -10, -12 and TNFα (each P<0.03). Immunostaining revealed significantly higher number of DCs, T-cells and macrophages (each P<0.002) in infarcted than control myocardium. We show that circulating DCPs are significantly reduced in AMI, with a pronounced reduction in STEMI patients. This was accompanied by a significant increase of inflammatory serum cytokines in patients with AMI. Immunohistochemical analysis unravelled that the reduction of circulating DCPs might be due to recruitment into the infarcted myocardium.
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Research Article|
May 29 2012
Recruitment of circulating dendritic cell precursors into the infarcted myocardium and pro-inflammatory response in acute myocardial infarction
Daniel Kretzschmar;
*Department of Internal Medicine I, Division of Cardiology and Intensive Care Medicine, University Hospital Jena, Jena, Germany
Correspondence: Dr Daniel Kretzschmar (email [email protected]).
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Stefan Betge;
Stefan Betge
*Department of Internal Medicine I, Division of Cardiology and Intensive Care Medicine, University Hospital Jena, Jena, Germany
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Alexander Windisch;
Alexander Windisch
*Department of Internal Medicine I, Division of Cardiology and Intensive Care Medicine, University Hospital Jena, Jena, Germany
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Rudin Pistulli;
Rudin Pistulli
*Department of Internal Medicine I, Division of Cardiology and Intensive Care Medicine, University Hospital Jena, Jena, Germany
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Ilonka Rohm;
Ilonka Rohm
*Department of Internal Medicine I, Division of Cardiology and Intensive Care Medicine, University Hospital Jena, Jena, Germany
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Michael Fritzenwanger;
Michael Fritzenwanger
*Department of Internal Medicine I, Division of Cardiology and Intensive Care Medicine, University Hospital Jena, Jena, Germany
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Christian Jung;
Christian Jung
*Department of Internal Medicine I, Division of Cardiology and Intensive Care Medicine, University Hospital Jena, Jena, Germany
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Katja Schubert;
Katja Schubert
*Department of Internal Medicine I, Division of Cardiology and Intensive Care Medicine, University Hospital Jena, Jena, Germany
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Bernhard Theis;
Bernhard Theis
†Institute of Pathology, University Hospital Jena, Jena, Germany
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Iver Petersen;
Iver Petersen
†Institute of Pathology, University Hospital Jena, Jena, Germany
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Stefanie Drobnik;
Stefanie Drobnik
‡Institute of Legal Medicine, University Hospital Jena, Jena, Germany
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Gita Mall;
Gita Mall
‡Institute of Legal Medicine, University Hospital Jena, Jena, Germany
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Hans-Reiner Figulla;
Hans-Reiner Figulla
*Department of Internal Medicine I, Division of Cardiology and Intensive Care Medicine, University Hospital Jena, Jena, Germany
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Atilla Yilmaz
Atilla Yilmaz
*Department of Internal Medicine I, Division of Cardiology and Intensive Care Medicine, University Hospital Jena, Jena, Germany
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Publisher: Portland Press Ltd
Received:
November 02 2011
Revision Received:
March 05 2012
Accepted:
April 11 2012
Accepted Manuscript online:
April 11 2012
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© The Authors Journal compilation © 2012 Biochemical Society
2012
Clin Sci (Lond) (2012) 123 (6): 387–398.
Article history
Received:
November 02 2011
Revision Received:
March 05 2012
Accepted:
April 11 2012
Accepted Manuscript online:
April 11 2012
Citation
Daniel Kretzschmar, Stefan Betge, Alexander Windisch, Rudin Pistulli, Ilonka Rohm, Michael Fritzenwanger, Christian Jung, Katja Schubert, Bernhard Theis, Iver Petersen, Stefanie Drobnik, Gita Mall, Hans-Reiner Figulla, Atilla Yilmaz; Recruitment of circulating dendritic cell precursors into the infarcted myocardium and pro-inflammatory response in acute myocardial infarction. Clin Sci (Lond) 1 September 2012; 123 (6): 387–398. doi: https://doi.org/10.1042/CS20110561
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