PE (pre-eclampsia), a pregnancy-specific disorder, is characterized by increased trophoblast cell death and deficient trophoblast invasion and reduced trophoblast-mediated remodelling of spiral arteries. The present study was performed to determine the function of miR-29b (microRNA-29b) in trophoblast cells and its underlying role in the pathogenesis of PE. The prediction of miR-29b target genes was performed using computer-based programs, including Targetscan, Pictar and miRBase. The function of these target genes was analysed further by gene ontology (GO). The effects of miR-29b on apoptosis, and invasion and angiogenesis of trophoblast cell lines (HTR-8/SVneo, BeWo and JAR) were examined by flow cytometry and Matrigel assay respectively. We found that miR-29b induced apoptosis and inhibited invasion and angiogenesis of trophoblast cells. Further studies confirmed that miR-29b regulated the expression of MCL1 (myeloid cell leukaemia sequence 1), MMP2 (encoding matrix metallproteinase 2), VEGFA (vascular endothelial growth factor A) and ITGB1 (integrin β1) genes by directly binding to their 3′-UTRs (untranslated regions). Moreover, we identified that there was an inverse correlation between miR-29b and its target genes in subjects with PE. Taken together, these findings support a novel role for miR-29b in invasion, apoptosis and angiogenesis of trophoblast cells, and miR-29b may become a new potential therapeutic target for PE.
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Research Article|
September 07 2012
microRNA-29b contributes to pre-eclampsia through its effects on apoptosis, invasion and angiogenesis of trophoblast cells
Pengfei Li;
Pengfei Li
*Immunology and Reproductive Biology Lab, Medical School & State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China
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Wei Guo;
Wei Guo
*Immunology and Reproductive Biology Lab, Medical School & State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China
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Leilei Du;
Leilei Du
*Immunology and Reproductive Biology Lab, Medical School & State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China
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Junli Zhao;
Junli Zhao
*Immunology and Reproductive Biology Lab, Medical School & State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China
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Yaping Wang;
Yaping Wang
*Immunology and Reproductive Biology Lab, Medical School & State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China
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Liu Liu;
Liu Liu
*Immunology and Reproductive Biology Lab, Medical School & State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China
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Yali Hu;
†The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008, China
‡Jiangsu Key Laboratory of Molecular Medicine, Nanjing University, Nanjing 210093, China
Dr Yayi Hou (email [email protected]) or Dr Yali Hu (email [email protected]).
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Yayi Hou
*Immunology and Reproductive Biology Lab, Medical School & State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China
‡Jiangsu Key Laboratory of Molecular Medicine, Nanjing University, Nanjing 210093, China
Dr Yayi Hou (email [email protected]) or Dr Yali Hu (email [email protected]).
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Publisher: Portland Press Ltd
Received:
March 05 2012
Revision Received:
May 18 2012
Accepted:
June 21 2012
Accepted Manuscript online:
June 21 2012
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© The Authors Journal compilation © 2013 Biochemical Society
2013
Clin Sci (Lond) (2013) 124 (1): 27–40.
Article history
Received:
March 05 2012
Revision Received:
May 18 2012
Accepted:
June 21 2012
Accepted Manuscript online:
June 21 2012
Citation
Pengfei Li, Wei Guo, Leilei Du, Junli Zhao, Yaping Wang, Liu Liu, Yali Hu, Yayi Hou; microRNA-29b contributes to pre-eclampsia through its effects on apoptosis, invasion and angiogenesis of trophoblast cells. Clin Sci (Lond) 1 January 2013; 124 (1): 27–40. doi: https://doi.org/10.1042/CS20120121
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