Sorafenib is the standard treatment for patients with advanced hepatocellular carcinoma (HCC). However, the median overall survival (OS) benefit is only ~3 months, and sufficient biomarkers predicting treatment response are not available. The aim of the present study was to evaluate miRNA expression patterns from HCC tissue biopsies as potential biomarkers in patients under sorafenib treatment. Nineteen patients with advanced HCC treated with sorafenib were included. RNA was extracted from formalin-fixed paraffin-embedded (FFPE) liver biopsies. miRNA expression profiling of 818 mature miRNAs was performed using GeneChip® miRNA Array 2.0 (Affymetrix). Global miRNA patterns were assessed using unsupervised hierarchical clustering analysis (UCA), and specific miRNAs with correlation with disease control rate (DCR) or good OS were evaluated by pairwise supervised analyses. UCA divided the patients into three distinct groups by their miRNA expression patterns. However, DCR or OS did not correlate with these sub-groups. We have identified several miRNAs that correlated with either DCR or OS (P<0.05). However, with correction for multiple testing, these results did not reach statistical significance in this small cohort. Global miRNA analysis from very low input RNA deriving from liver biopsies showed distinctive clustering of molecular sub-groups in patients with intermediate and advanced HCC. Clinical response including OS under sorafenib did not correlate with global miRNA expression patterns, but we have identified candidate miRNAs for the prediction of DCR and OS to be evaluated in prospective studies and larger patient cohorts.
Skip Nav Destination
Close
Article navigation
January 2015
- Cover Image
- PDF Icon PDF LinkTable of Contents
Research Article|
August 27 2014
Feasibility of global miRNA analysis from fine-needle biopsy FFPE material in patients with hepatocellular carcinoma treated with sorafenib
Jan Peveling-Oberhag
;
*Medizinische Klinik 1, Universitätsklinikum Frankfurt, Goethe-Universität, Theodor-Stern-Kai 7, 60590 Frankfurt am Main, Germany
Correspondence: Dr Jan Peveling-Oberhag (email jan.peveling-oberhag@kgu.de).
Search for other works by this author on:
Claudia Döring
;
Claudia Döring
†Senckenbergisches Institut für Pathologie, Universitätsklinikum Frankfurt, Goethe-Universität, Theodor-Stern-Kai 7, 60590 Frankfurt am Main, Germany
Search for other works by this author on:
Sylvia Hartmann
;
Sylvia Hartmann
†Senckenbergisches Institut für Pathologie, Universitätsklinikum Frankfurt, Goethe-Universität, Theodor-Stern-Kai 7, 60590 Frankfurt am Main, Germany
Search for other works by this author on:
Natalie Filmann
;
Natalie Filmann
‡Institut für Biostatistik und Mathematische Modellierung, Fachbereich Medizin, Goethe-Universität, Theodor-Stern-Kai 7, 60590 Frankfurt am Main, Germany
Search for other works by this author on:
Angelika Mertens
;
Angelika Mertens
*Medizinische Klinik 1, Universitätsklinikum Frankfurt, Goethe-Universität, Theodor-Stern-Kai 7, 60590 Frankfurt am Main, Germany
Search for other works by this author on:
Albrecht Piiper
;
Albrecht Piiper
*Medizinische Klinik 1, Universitätsklinikum Frankfurt, Goethe-Universität, Theodor-Stern-Kai 7, 60590 Frankfurt am Main, Germany
Search for other works by this author on:
Eva Herrmann
;
Eva Herrmann
‡Institut für Biostatistik und Mathematische Modellierung, Fachbereich Medizin, Goethe-Universität, Theodor-Stern-Kai 7, 60590 Frankfurt am Main, Germany
Search for other works by this author on:
Martin-Leo Hansmann
;
Martin-Leo Hansmann
†Senckenbergisches Institut für Pathologie, Universitätsklinikum Frankfurt, Goethe-Universität, Theodor-Stern-Kai 7, 60590 Frankfurt am Main, Germany
Search for other works by this author on:
Stefan Zeuzem
;
Stefan Zeuzem
*Medizinische Klinik 1, Universitätsklinikum Frankfurt, Goethe-Universität, Theodor-Stern-Kai 7, 60590 Frankfurt am Main, Germany
Search for other works by this author on:
Jörg Trojan
;
Jörg Trojan
1
*Medizinische Klinik 1, Universitätsklinikum Frankfurt, Goethe-Universität, Theodor-Stern-Kai 7, 60590 Frankfurt am Main, Germany
Search for other works by this author on:
Martin-Walter Welker
Martin-Walter Welker
1
*Medizinische Klinik 1, Universitätsklinikum Frankfurt, Goethe-Universität, Theodor-Stern-Kai 7, 60590 Frankfurt am Main, Germany
Search for other works by this author on:
Clin Sci (Lond) (2015) 128 (1): 29–37.
Article history
Received:
January 02 2014
Revision Received:
May 22 2014
Accepted:
June 24 2014
Accepted Manuscript online:
June 24 2014
Citation
Jan Peveling-Oberhag, Claudia Döring, Sylvia Hartmann, Natalie Filmann, Angelika Mertens, Albrecht Piiper, Eva Herrmann, Martin-Leo Hansmann, Stefan Zeuzem, Jörg Trojan, Martin-Walter Welker; Feasibility of global miRNA analysis from fine-needle biopsy FFPE material in patients with hepatocellular carcinoma treated with sorafenib. Clin Sci (Lond) 1 January 2015; 128 (1): 29–37. doi: https://doi.org/10.1042/CS20140007
Download citation file:
Close
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Biochemical Society Member Sign in
Sign InSign in via your Institution
Sign in via your InstitutionGet Access To This Article
Cited By
Related Articles
Radiofrequency ablation suppresses distant tumour growth in a novel rat model of multifocal hepatocellular carcinoma
Clin Sci (Lond) (October,2013)
UBE2C functions as a potential oncogene by enhancing cell proliferation, migration, invasion, and drug resistance in hepatocellular carcinoma cells
Biosci Rep (April,2019)
Targeting vessels to treat hepatocellular carcinoma
Clin Sci (Lond) (February,2008)
Sorafenib treatment improves hepatopulmonary syndrome in rats with biliary cirrhosis
Clin Sci (Lond) (December,2012)