Microparticles (MPs) could be considered biomarkers of cell damage and activation as well as novel signalling structures. Since rheumatoid arthritis (RA) is characterized by immune and endothelial activation, the main aim of the present study was to analyse MP counts in RA patients. Citrated-blood samples were obtained from 114 RA patients, 33 healthy controls (HC) and 72 individuals with marked cardiovascular (CV) risk without autoimmune manifestations (CVR). MPs were analysed in platelet-poor plasma (PPP) and different subsets were identified by their surface markers: platelet- (CD41+), endothelial- (CD146+), granulocyte- (CD66+), monocyte- (CD14+) and Tang- (CD3+CD31+) derived. Disease activity score (DAS28), clinical and immunological parameters as well as traditional CV risk factors (diabetes, hypertension, dyslipidaemia and obesity) were registered from clinical records and all data were integrated using Principal Component Analysis (PCA). Absolute MP number was increased in RA patients compared with HC and positively correlated with traditional CV risk factors, similar to that of CVR subjects. In addition, frequency of the different MP subsets was different in RA patients and significantly associated with disease features. Moreover, in vitro assays revealed that MPs isolated from RA patients were able to promote endothelial activation and exhibited detrimental effects on human microvascular endothelial cells (HMEC-I) endothelial cell functionality. Circulating MPs from RA patients displayed quantitative and qualitative alterations that are the result of both disease-specific and traditional CV risk factors. Accordingly, this MP pool exhibited in vitro detrimental effects on endothelial cells, thus supporting their role as biomarkers of vascular damage.
Skip Nav Destination
Article navigation
Research Article|
December 16 2014
Altered profile of circulating microparticles in rheumatoid arthritis patients
Javier Rodríguez-Carrio;
Javier Rodríguez-Carrio
*Area of Immunology, Department of Functional Biology, University of Oviedo, Asturias, Spain
Search for other works by this author on:
Mercedes Alperi-López;
Mercedes Alperi-López
†Department of Rheumatology, Hospital Universitario Central de Asturias, Asturias, Spain
Search for other works by this author on:
Patricia López;
Patricia López
*Area of Immunology, Department of Functional Biology, University of Oviedo, Asturias, Spain
Search for other works by this author on:
Sara Alonso-Castro;
Sara Alonso-Castro
†Department of Rheumatology, Hospital Universitario Central de Asturias, Asturias, Spain
Search for other works by this author on:
Santiago R. Carro-Esteban;
Santiago R. Carro-Esteban
*Area of Immunology, Department of Functional Biology, University of Oviedo, Asturias, Spain
Search for other works by this author on:
Francisco J. Ballina-García;
Francisco J. Ballina-García
†Department of Rheumatology, Hospital Universitario Central de Asturias, Asturias, Spain
Search for other works by this author on:
Ana Suárez
*Area of Immunology, Department of Functional Biology, University of Oviedo, Asturias, Spain
Correspondence: Dr Ana Suárez (email [email protected]).
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
October 23 2014
Accepted:
November 04 2014
Accepted Manuscript online:
November 04 2014
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© The Authors Journal compilation © 2015 Biochemical Society
2015
Clin Sci (Lond) (2015) 128 (7): 437–448.
Article history
Received:
October 23 2014
Accepted:
November 04 2014
Accepted Manuscript online:
November 04 2014
Citation
Javier Rodríguez-Carrio, Mercedes Alperi-López, Patricia López, Sara Alonso-Castro, Santiago R. Carro-Esteban, Francisco J. Ballina-García, Ana Suárez; Altered profile of circulating microparticles in rheumatoid arthritis patients. Clin Sci (Lond) 1 April 2015; 128 (7): 437–448. doi: https://doi.org/10.1042/CS20140675
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Biochemical Society Member Sign in
Sign InSign in via your Institution
Sign in via your InstitutionGet Access To This Article
Open Access for all
We offer compliant routes for all authors from 2025. With library support, there will be no author nor reader charges in 5 journals. Check here |
![]() |