To compare the cumulative (3-day) effect of prolonged sitting on metabolic responses during a mixed meal tolerance test (MTT), with sitting that is regularly interrupted with brief bouts of light-intensity walking. Overweight/obese adults (n=19) were recruited for a randomized, 3-day, outpatient, cross-over trial involving: (1) 7-h days of uninterrupted sitting (SIT); and (2) 7-h days of sitting with light-intensity activity breaks [BREAKS; 2-min of treadmill walking (3.2 km/h) every 20 min (total: 17 breaks/day)]. On days 1 and 3, participants underwent a MTT (75 g of carbohydrate, 50 g of fat) and the incremental area under the curve (iAUC) was calculated from hourly blood samples. Generalized estimating equation (GEE) models were adjusted for gender, body mass index (BMI), energy intake, treatment order and pre-prandial values to determine effects of time, condition and time × condition. The glucose iAUC was 1.3±0.5 and 1.5±0.5 mmol·h·l−1 (mean differences ± S.E.M.) higher in SIT compared with BREAKS on days 1 and 3 respectively (condition effect: P=0.001), with no effect of time (P=0.48) or time × condition (P=0.8). The insulin iAUC was also higher on both days in SIT (day 1: ∆151±73, day 3: ∆91±73 pmol·h·l−1, P=0.01), with no effect of time (P=0.52) or time × condition (P=0.71). There was no between-treatment difference in triglycerides (triacylglycerols) iAUC. There were significant between-condition effects but no temporal change in metabolic responses to MTT, indicating that breaking up of sitting over 3 days sustains, but does not enhance, the lowering of postprandial glucose and insulin.
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April 24 2015
Breaking up of prolonged sitting over three days sustains, but does not enhance, lowering of postprandial plasma glucose and insulin in overweight and obese adults
Robyn N. Larsen;
*Physical Activity Laboratory, Baker IDI Heart and Diabetes Institute, Melbourne, Victoria 3004, Australia
Correspondence: Dr Robyn Larsen (email [email protected]).
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Bronwyn A. Kingwell;
Bronwyn A. Kingwell
†Metabolic and Vascular Physiology Laboratory, Baker IDI Heart and Diabetes Institute, Melbourne, Victoria 3004, Australia
║Department of Medicine, Monash University, Melbourne, Victoria 3800, Australia
¶Department of Physiology, Monash University, Melbourne, Victoria 3800, Australia
**Department of Physiology, The University of Melbourne, Parkville, Victoria 3010, Australia
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Carol Robinson;
Carol Robinson
*Physical Activity Laboratory, Baker IDI Heart and Diabetes Institute, Melbourne, Victoria 3004, Australia
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Louise Hammond;
Louise Hammond
*Physical Activity Laboratory, Baker IDI Heart and Diabetes Institute, Melbourne, Victoria 3004, Australia
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Ester Cerin;
Ester Cerin
††School of Exercise and Nutrition Sciences, Deakin University, 221 Burwood Hwy, Burwood, Victoria 3125, Australia
‡‡Institute of Human Performance, The University of Hong Kong, Pokfulam, Hong Kong SAR, China
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Jonathan E. Shaw;
Jonathan E. Shaw
‡Clinical Diabetes Laboratory, Baker IDI Heart and Diabetes Institute, Melbourne, Victoria 3004, Australia
§§Department of Epidemiology and Preventive Medicine, Monash University, Melbourne, Victoria 3800, Australia
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Genevieve N. Healy;
Genevieve N. Healy
*Physical Activity Laboratory, Baker IDI Heart and Diabetes Institute, Melbourne, Victoria 3004, Australia
║║School of Population Health, The University of Queensland, St Lucia, Queensland 4072, Australia
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Marc T. Hamilton;
Marc T. Hamilton
¶Department of Physiology, Monash University, Melbourne, Victoria 3800, Australia
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Neville Owen;
Neville Owen
§Behavioural Epidemiology Laboratory, Baker IDI Heart and Diabetes Institute, Melbourne, Victoria 3004, Australia
║Department of Medicine, Monash University, Melbourne, Victoria 3800, Australia
║║School of Population Health, The University of Queensland, St Lucia, Queensland 4072, Australia
¶¶Melbourne School of Population Health, The University of Melbourne, Melbourne, Victoria 3010, Australia
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David W. Dunstan
David W. Dunstan
*Physical Activity Laboratory, Baker IDI Heart and Diabetes Institute, Melbourne, Victoria 3004, Australia
║Department of Medicine, Monash University, Melbourne, Victoria 3800, Australia
††School of Exercise and Nutrition Sciences, Deakin University, 221 Burwood Hwy, Burwood, Victoria 3125, Australia
§§Department of Epidemiology and Preventive Medicine, Monash University, Melbourne, Victoria 3800, Australia
║║School of Population Health, The University of Queensland, St Lucia, Queensland 4072, Australia
***Inactivity Physiology Department, Pennington Biomedical Research Center, 6400 Perkins Rd, Baton Rouge, LA 70808, U.S.A.
†††School of Sport Science, Exercise and Health, The University of Western Australia, 35 Stirling Hwy, Crawley, Perth, Western Australia 6009, Australia
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Publisher: Portland Press Ltd
Received:
December 08 2014
Revision Received:
February 23 2015
Accepted:
March 03 2015
Accepted Manuscript online:
March 03 2015
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© The Authors Journal compilation © 2015 Biochemical Society
2015
Clin Sci (Lond) (2015) 129 (2): 117–127.
Article history
Received:
December 08 2014
Revision Received:
February 23 2015
Accepted:
March 03 2015
Accepted Manuscript online:
March 03 2015
Citation
Robyn N. Larsen, Bronwyn A. Kingwell, Carol Robinson, Louise Hammond, Ester Cerin, Jonathan E. Shaw, Genevieve N. Healy, Marc T. Hamilton, Neville Owen, David W. Dunstan; Breaking up of prolonged sitting over three days sustains, but does not enhance, lowering of postprandial plasma glucose and insulin in overweight and obese adults. Clin Sci (Lond) 1 July 2015; 129 (2): 117–127. doi: https://doi.org/10.1042/CS20140790
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