Plasma lactate is widely used as a biomarker in critical illness. The aims of the present study were to elucidate the usefulness of a three-compartment model for muscle lactate kinetics in humans and to characterize the response to an exogenous adrenaline challenge. Repeated blood samples from artery and femoral vein together with blood flow measurements and muscle biopsies were obtained from healthy male volunteers (n=8) at baseline and during an adrenaline infusion. Concentrations of lactate and enrichment of [13C]lactate were measured and kinetics calculated. Mitochondrial activity, glycogen concentration, oxygen uptake and CO2 release were assessed. The adrenaline challenge increased plasma lactate 4-fold as a result of a greater increase in the rate of appearance (Ra) than the increase in the rate of disappearance (Rd). Leg muscle net release of lactate increased 3.5-fold, whereas intramuscular production had a high variation but did not change. Mitochondrial state 3 respiration increased by 30%. Glycogen concentration, oxygen uptake and CO2 production remained unchanged. In conclusion a three-compartment model gives additional information to the two-compartment model but, due to its larger variation and invasive muscle biopsy, it is less likely to become a regularly used tool in clinical research. Hyperlactataemia in response to adrenergic stimuli was driven by an elevated lactate release from skeletal muscle most probably due to a redirection of a high intramuscular turnover rather than an increased production.
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Research Article|
May 27 2015
Lactate kinetics and mitochondrial respiration in skeletal muscle of healthy humans under influence of adrenaline
Jonathan Grip;
*Clintec, Department of Anesthesiology and Intensive Care, Karolinska Institutet and Karolinska University Hospital, 141 86 Huddinge, Sweden
Correspondence: Dr Jonathan Grip (email [email protected]).
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Towe Jakobsson;
Towe Jakobsson
*Clintec, Department of Anesthesiology and Intensive Care, Karolinska Institutet and Karolinska University Hospital, 141 86 Huddinge, Sweden
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Christina Hebert;
Christina Hebert
*Clintec, Department of Anesthesiology and Intensive Care, Karolinska Institutet and Karolinska University Hospital, 141 86 Huddinge, Sweden
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Maria Klaude;
Maria Klaude
*Clintec, Department of Anesthesiology and Intensive Care, Karolinska Institutet and Karolinska University Hospital, 141 86 Huddinge, Sweden
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Gustaf Sandström;
Gustaf Sandström
*Clintec, Department of Anesthesiology and Intensive Care, Karolinska Institutet and Karolinska University Hospital, 141 86 Huddinge, Sweden
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Jan Wernerman;
Jan Wernerman
*Clintec, Department of Anesthesiology and Intensive Care, Karolinska Institutet and Karolinska University Hospital, 141 86 Huddinge, Sweden
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Olav Rooyackers
Olav Rooyackers
*Clintec, Department of Anesthesiology and Intensive Care, Karolinska Institutet and Karolinska University Hospital, 141 86 Huddinge, Sweden
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Publisher: Portland Press Ltd
Received:
July 28 2014
Revision Received:
March 16 2015
Accepted:
April 01 2015
Accepted Manuscript online:
April 01 2015
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© 2015 Authors; published by Portland Press Limited
2015
Clin Sci (Lond) (2015) 129 (4): 375–384.
Article history
Received:
July 28 2014
Revision Received:
March 16 2015
Accepted:
April 01 2015
Accepted Manuscript online:
April 01 2015
Citation
Jonathan Grip, Towe Jakobsson, Christina Hebert, Maria Klaude, Gustaf Sandström, Jan Wernerman, Olav Rooyackers; Lactate kinetics and mitochondrial respiration in skeletal muscle of healthy humans under influence of adrenaline. Clin Sci (Lond) 1 August 2015; 129 (4): 375–384. doi: https://doi.org/10.1042/CS20140448
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