Proprotein convertase subtilisin/kexin type 9 (PCSK9) regulates low-density lipoprotein (LDL) cholesterol (LDL-C) metabolism by targeting LDL receptors for degradation. Statins increase serum PCSK9 concentration limiting the potential of statins to reduce LDL-C, whereas ezetimibe, inhibitor of cholesterol absorption, has ambiguous effects on circulating PCSK9 levels. Plant stanols also reduce cholesterol absorption, but their effect on serum PCSK9 concentration is not known. Therefore, we performed a controlled, randomized, double-blind study, in which 92 normo- to moderately hypercholesterolaemic subjects (35 males and 57 females) consumed vegetable-oil spread 20 g/day enriched (plant stanol group, n=46) or not (control group, n=46) with plant stanols 3 g/day as ester for 6 months. Fasting blood samples were drawn at baseline and at the end of the study. Serum PCSK9 concentration was analysed with Quantikine Elisa Immunoassay, serum and lipoprotein lipids enzymatically and serum non-cholesterol sterols with GLC. At baseline, PCSK9 concentration varied from 91 to 716 ng/ml with a mean value of 278±11 (S.E.M.) ng/ml with no gender difference. It correlated with serum and LDL-C, serum triglycerides, age, body mass index (BMI) and plasma glucose concentration, but not with variables of cholesterol metabolism when adjusted to serum cholesterol. Plant stanols reduced LDL-C by 10% from controls (P<0.05), but PCSK9 levels were unchanged and did not differ between the groups. In conclusion, the present study demonstrated for the first time that inhibition of cholesterol absorption with plant stanol esters did not affect serum PCSK9 concentration. Thus, plant stanol esters provide an efficient dietary means to lower LDL-C without interfering with the PCSK9 metabolism and in this regard the LDL receptor-mediated cellular cholesterol uptake and removal.
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Research Article|
June 11 2015
Serum proprotein convertase subtilisin/kexin type 9 concentration is not increased by plant stanol ester consumption in normo- to moderately hypercholesterolaemic non-obese subjects. The BLOOD FLOW intervention study
Piia Simonen;
*University of Helsinki and Helsinki University Central Hospital, Heart and Lung Center, Helsinki, P.O. BOX 700, 00029 HUS, Finland
Correspondence: Dr Piia Simonen (email [email protected]).
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Ulf-Håkan Stenman;
Ulf-Håkan Stenman
†University of Helsinki and Helsinki University Central Hospital, Clinical Chemistry, Helsinki, P.O. BOX 700, 00029 HUS, Finland
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Helena Gylling
Helena Gylling
‡University of Helsinki and Helsinki University Central Hospital, Internal Medicine, Helsinki, P.O. BOX 700, 00029 HUS, Finland
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Publisher: Portland Press Ltd
Received:
March 12 2015
Revision Received:
April 07 2015
Accepted:
April 10 2015
Accepted Manuscript online:
April 10 2015
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© 2015 Authors; published by Portland Press Limited
2015
Clin Sci (Lond) (2015) 129 (5): 439–446.
Article history
Received:
March 12 2015
Revision Received:
April 07 2015
Accepted:
April 10 2015
Accepted Manuscript online:
April 10 2015
Citation
Piia Simonen, Ulf-Håkan Stenman, Helena Gylling; Serum proprotein convertase subtilisin/kexin type 9 concentration is not increased by plant stanol ester consumption in normo- to moderately hypercholesterolaemic non-obese subjects. The BLOOD FLOW intervention study. Clin Sci (Lond) 1 September 2015; 129 (5): 439–446. doi: https://doi.org/10.1042/CS20150193
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