Long non-coding RNAs (lncRNAs) have been reported to be involved in the pathogenesis of cardiovascular disease (CVD), but whether circulating lncRNAs can serve as a coronary artery disease (CAD), biomarker is not known. The present study screened lncRNAs by microarray analysis in the plasma from CAD patients and control individuals and found that 265 lncRNAs were differentially expressed. To find specific lncRNAs as possible CAD biomarker candidates, we used the following criteria for 174 up-regulated lncRNAs: signal intensity ≥8, fold change >2.5 and P<0.005. According to these criteria, five intergenic lncRNAs were identified. After validation by quantitative PCR (qPCR), one lncRNA was excluded from the candidate list. The remaining four lncRNAs were independently validated in another population of 20 CAD patients and 20 control individuals. Receiver operating characteristic (ROC) curve analysis showed that lncRNA AC100865.1 (referred to as CoroMarker) was the best of these lncRNAs. CoroMarker levels were also stable in plasma. The predictive value of CoroMarker was further assessed in a larger cohort with 221 CAD patients and 187 control individuals. Using a diagnostic model with Fisher's criteria, taking the risk factors into account, the optimal sensitivity of CoroMarker for CAD increased from 68.29% to 78.05%, whereas the specificity decreased slightly from 91.89% to 86.49%. CoroMarker was stable in plasma because it was mainly in the extracellular vesicles (EVs), probably from monocytes. We conclude that CoroMarker is a stable, sensitive and specific biomarker for CAD.
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Research Article|
July 22 2015
Plasma long non-coding RNA, CoroMarker, a novel biomarker for diagnosis of coronary artery disease
Yujia Yang;
Yujia Yang
1
*Department of Cardiology, Daping Hospital, Third Military Medical University, Chongqing, China
†Chongqing Institute of Cardiology, Chongqing, China
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Yue Cai;
Yue Cai
1
*Department of Cardiology, Daping Hospital, Third Military Medical University, Chongqing, China
†Chongqing Institute of Cardiology, Chongqing, China
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Gengze Wu;
Gengze Wu
*Department of Cardiology, Daping Hospital, Third Military Medical University, Chongqing, China
†Chongqing Institute of Cardiology, Chongqing, China
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Xinjian Chen;
Xinjian Chen
*Department of Cardiology, Daping Hospital, Third Military Medical University, Chongqing, China
†Chongqing Institute of Cardiology, Chongqing, China
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Yukai Liu;
Yukai Liu
*Department of Cardiology, Daping Hospital, Third Military Medical University, Chongqing, China
†Chongqing Institute of Cardiology, Chongqing, China
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Xinquan Wang;
Xinquan Wang
*Department of Cardiology, Daping Hospital, Third Military Medical University, Chongqing, China
†Chongqing Institute of Cardiology, Chongqing, China
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Junyi Yu;
Junyi Yu
*Department of Cardiology, Daping Hospital, Third Military Medical University, Chongqing, China
†Chongqing Institute of Cardiology, Chongqing, China
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Chuanwei Li;
Chuanwei Li
*Department of Cardiology, Daping Hospital, Third Military Medical University, Chongqing, China
†Chongqing Institute of Cardiology, Chongqing, China
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Xiongwen Chen;
Xiongwen Chen
*Department of Cardiology, Daping Hospital, Third Military Medical University, Chongqing, China
‡Department of Physiology and Cardiovascular Research Center, Temple University School of Medicine, Philadelphia, PA, U.S.A.
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Pedro A. Jose;
Pedro A. Jose
§Division of Nephrology, Department of Medicine, University of Maryland School of Medicine, Baltimore, MD, U.S.A.
¶Department of Physiology, University of Maryland School of Medicine, Baltimore, MD, U.S.A.
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Lin Zhou;
*Department of Cardiology, Daping Hospital, Third Military Medical University, Chongqing, China
†Chongqing Institute of Cardiology, Chongqing, China
Correspondence: Dr Chunyu Zeng ([email protected]) and Dr Lin Zhou ([email protected])
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Chunyu Zeng
*Department of Cardiology, Daping Hospital, Third Military Medical University, Chongqing, China
†Chongqing Institute of Cardiology, Chongqing, China
Correspondence: Dr Chunyu Zeng ([email protected]) and Dr Lin Zhou ([email protected])
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Publisher: Portland Press Ltd
Received:
February 11 2015
Revision Received:
May 26 2015
Accepted:
June 05 2015
Accepted Manuscript online:
June 10 2015
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© 2015 Authors; published by Portland Press Limited
2015
Clin Sci (Lond) (2015) 129 (8): 675–685.
Article history
Received:
February 11 2015
Revision Received:
May 26 2015
Accepted:
June 05 2015
Accepted Manuscript online:
June 10 2015
Citation
Yujia Yang, Yue Cai, Gengze Wu, Xinjian Chen, Yukai Liu, Xinquan Wang, Junyi Yu, Chuanwei Li, Xiongwen Chen, Pedro A. Jose, Lin Zhou, Chunyu Zeng; Plasma long non-coding RNA, CoroMarker, a novel biomarker for diagnosis of coronary artery disease. Clin Sci (Lond) 1 October 2015; 129 (8): 675–685. doi: https://doi.org/10.1042/CS20150121
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