During sepsis, endothelial barrier dysfunction contributes to cardiovascular failure, mainly through the release of oxidative metabolites by penetrant leukocytes. We reported the non-muscular isoform of myosin light chain kinase (nmMLCK) playing a pivotal role in endotoxin shock injury associated with oxidative and nitrative stresses, and vascular hyporeactivity. The present study was aimed at understanding the molecular mechanism of lipopolysaccharide (LPS)-induced vascular alterations as well as studying a probable functional association of nmMLCK with nuclear factor κ-light-chain enhancer of activated B cells (NF-κB). Aortic rings from mice were exposed in vitro to LPS and, then, vascular reactivity was measured. Human aortic endothelial cells (HAoECs) were incubated with LPS, and interaction of nmMLCK with NF-κB was analysed. We provide evidence that nmMLCK deletion prevents vascular hyporeactivity induced by in vitro LPS treatment but not endothelial dysfunction in the aorta. Deletion of nmMLCK inhibits LPS-induced NF-κB activation and increases nitric oxide (NO) release via induction of inducible NO synthase (iNOS) within the vascular wall. Also, removal of endothelium prevented both NF-κB and iNOS expression in aortic rings. Among the proinflammatory factors released by LPS-treated endothelial cells, interleukin-6 accounts for the induction of iNOS on smooth muscle cells in response to LPS. Of particular interest is the demonstration that, in HAoECs, LPS-induced NF-κB activation occurs via increased MLCK activity sensitive to the MLCK inhibitor, ML-7, and physical interactions between nmMLCK and NF-κB. We report for the first time on NF-κB as a novel partner of nmMLCK within endothelial cells. The present study demonstrates a pivotal role of nmMLCK in vascular inflammatory pathologies.
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July 22 2015
Interaction in endothelium of non-muscular myosin light-chain kinase and the NF-κB pathway is critical to lipopolysaccharide-induced vascular hyporeactivity
Sylvain Recoquillon;
Sylvain Recoquillon
*LUNAM Université, INSERM, UMR 1063, Angers, France
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Nunzia Carusio;
Nunzia Carusio
*LUNAM Université, INSERM, UMR 1063, Angers, France
†Seconda Università di Napoli, Dipartimento di Medicina Sperimentale, Naples, Italy
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Anne-Hélène Lagrue-Lakhal;
Anne-Hélène Lagrue-Lakhal
*LUNAM Université, INSERM, UMR 1063, Angers, France
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Simon Tual-Chalot;
Simon Tual-Chalot
*LUNAM Université, INSERM, UMR 1063, Angers, France
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Amelia Filippelli;
Amelia Filippelli
†Seconda Università di Napoli, Dipartimento di Medicina Sperimentale, Naples, Italy
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Ramaroson Andriantsitohaina;
Ramaroson Andriantsitohaina
*LUNAM Université, INSERM, UMR 1063, Angers, France
‡Centre Hospitalo-Universitaire d’Angers, Angers, France
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M. Carmen Martinez
*LUNAM Université, INSERM, UMR 1063, Angers, France
‡Centre Hospitalo-Universitaire d’Angers, Angers, France
Correspondence: M. Carmen Martinez ([email protected]).
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Publisher: Portland Press Ltd
Received:
October 02 2014
Revision Received:
June 03 2015
Accepted:
June 15 2015
Accepted Manuscript online:
June 15 2015
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© 2015 Authors; published by Portland Press Limited
2015
Clin Sci (Lond) (2015) 129 (8): 687–698.
Article history
Received:
October 02 2014
Revision Received:
June 03 2015
Accepted:
June 15 2015
Accepted Manuscript online:
June 15 2015
Citation
Sylvain Recoquillon, Nunzia Carusio, Anne-Hélène Lagrue-Lakhal, Simon Tual-Chalot, Amelia Filippelli, Ramaroson Andriantsitohaina, M. Carmen Martinez; Interaction in endothelium of non-muscular myosin light-chain kinase and the NF-κB pathway is critical to lipopolysaccharide-induced vascular hyporeactivity. Clin Sci (Lond) 1 October 2015; 129 (8): 687–698. doi: https://doi.org/10.1042/CS20140625
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