Catecholamines and atrial natriuretic peptide (ANP) are major regulators of adipocyte lipolysis. Although obesity is characterized by catecholamine resistance in subcutaneous adipose tissue (SCAT), data on ANP lipolytic response and sensitivity in different adipose tissue (AT) depots of metabolically distinct humans are scarce. Ex vivo catecholamine- and ANP-induced lipolysis was investigated in adipocytes derived from SCAT and visceral AT (VAT) depot of lean (n=13) and obese men, with (n=11) or without (n=18) type 2 diabetes (HbA1c < or ≥ 6.5%). Underlying molecular mechanisms were examined by looking at functional receptors in the NP signalling pathway at the mRNA and protein level. Maximal ANP- and catecholamine-induced lipolysis in SCAT was blunted in obese type 2 diabetics compared with age-matched lean men whereas non-diabetic obese subjects showed intermediate responses. This blunted ANP-mediated lipolytic response was accompanied by lower mRNA and protein expression of the type-A natriuretic peptide (NP) receptor and higher mRNA but reduced protein expression of the scavenging type-C receptor. Maximal ANP-induced lipolysis was lower in VAT compared with SCAT but not different between groups. Collectively, our data show that both ANP- and catecholamine-mediated lipolysis is attenuated in SCAT of obese men with type 2 diabetes, and might be partially explained by NP receptor defects. Therefore, improving maximal ANP responsiveness in adipose tissue might be a potential novel strategy to improve obesity-associated metabolic complications.
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Sagittal section through the retina of a hypertensive dTGR rat: staining in blue (DAPI) of cell nuclei and in red GFAP (glial fibrillary acidic protein). The GFAP staining shows activated astrocytes in the ganglion cell layer of the retina. See pp. 1075–1088 for further details. Image kindly provided by Olaf Strauß.
Research Article|
May 23 2016
Attenuated atrial natriuretic peptide-mediated lipolysis in subcutaneous adipocytes of obese type 2 diabetic men
Kenneth Verboven;
*NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University Medical Centre+, Maastricht 6200 MD, The Netherlands
†REVAL Rehabilitation Research Center, BIOMED Biomedical Research Institute, Faculty of Medicine and Life Sciences, Hasselt University, Hasselt 3500, Belgium
Correspondence: Kenneth Verboven (email [email protected]).
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Dominique Hansen;
Dominique Hansen
†REVAL Rehabilitation Research Center, BIOMED Biomedical Research Institute, Faculty of Medicine and Life Sciences, Hasselt University, Hasselt 3500, Belgium
‡Heart Centre Hasselt, Jessa Hospital, Hasselt 3500, Belgium
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Cedric Moro;
Cedric Moro
§INSERM, UMR1048, Institute of Metabolic and Cardiovascular Diseases, Obesity Research Laboratory, Toulouse 31432 Cedex 4, France
║University of Toulouse, UMR1048, Paul Sabatier University, Toulouse 31432 Cedex 4, France
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Bert O. Eijnde;
Bert O. Eijnde
†REVAL Rehabilitation Research Center, BIOMED Biomedical Research Institute, Faculty of Medicine and Life Sciences, Hasselt University, Hasselt 3500, Belgium
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Nicole Hoebers;
Nicole Hoebers
*NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University Medical Centre+, Maastricht 6200 MD, The Netherlands
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Joep Knol;
Joep Knol
¶Department of Abdominal Surgery, Jessa Hospital, Hasselt 3500, Belgium
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Wim Bouckaert;
Wim Bouckaert
¶Department of Abdominal Surgery, Jessa Hospital, Hasselt 3500, Belgium
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Anne Dams;
Anne Dams
**Department of Abdominal Surgery, Hospital East-Limburg, Genk 3600, Belgium
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Ellen E. Blaak;
Ellen E. Blaak
1
*NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University Medical Centre+, Maastricht 6200 MD, The Netherlands
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Johan W.E. Jocken
Johan W.E. Jocken
1
*NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University Medical Centre+, Maastricht 6200 MD, The Netherlands
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Publisher: Portland Press Ltd
Received:
January 04 2016
Revision Received:
March 30 2016
Accepted:
April 01 2016
Accepted Manuscript online:
April 04 2016
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© 2016 The Author(s). published by Portland Press Limited on behalf of the Biochemical Society
2016
Clin Sci (Lond) (2016) 130 (13): 1105–1114.
Article history
Received:
January 04 2016
Revision Received:
March 30 2016
Accepted:
April 01 2016
Accepted Manuscript online:
April 04 2016
Citation
Kenneth Verboven, Dominique Hansen, Cedric Moro, Bert O. Eijnde, Nicole Hoebers, Joep Knol, Wim Bouckaert, Anne Dams, Ellen E. Blaak, Johan W.E. Jocken; Attenuated atrial natriuretic peptide-mediated lipolysis in subcutaneous adipocytes of obese type 2 diabetic men. Clin Sci (Lond) 1 July 2016; 130 (13): 1105–1114. doi: https://doi.org/10.1042/CS20160220
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