The present study aimed to: (i) identify the exogenous factors that allow in vitro differentiation of mouse spermatogonial stem cells (SSCs) from embryonic stem cells (ESCs); (ii) evaluate the effects of Sertoli cells in SSC enrichment; and (iii) assess the success of transplantation using in vitro differentiated SSCs in a mouse busulfan-treated azoospermia model. A 1-day-old embryoid body (EB) received 5 ng/ml of bone morphogenetic protein 4 (BMP4) for 4 days, 3 µM retinoic acid (RA) in a SIM mouse embryo-derived thioguanine and ouabain resistant (STO) co-culture system for 7 days, and was subsequently co-cultured for 2 days with Sertoli cells in the presence or absence of a leukaemia inhibitory factor (LIF), basic fibroblast growth factor (bFGF) and RA composition, and in the presence of these factors in simple culture medium. Higher viability, proliferation and germ cell gene expression were seen in the presence of the LIF, bFGF and RA composition, on top of Sertoli cells. Immunocytochemistry results showed higher CDH1 expression in this group. Sertoli co-culture had no effects on SSC proliferation. Eight weeks after transplantation, injected cells were observed at the base of the seminiferous tubules and in the recipient testes. The number of spermatogonia and the mass of the testes were higher in transplanted testes relative to the control group. It seems that transplantation of these cells can be useful in infertility treatment.
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September 2017
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A flurogold labeled-retina flatmount (front) representing surviving retinal ganglion cells, and the chemical structure of trimetazidine (background). In Clinical Science volume 131, issue 18, Wan et al. report that trimetazidine protects retinal ganglion cells against acute glaucoma via the Nrf2/Ho-1 pathway and propose it as a novel therapeutic agent; for details, see pages 2363-2375.
Research Article|
September 08 2017
Embryonic stem cell derived germ cells induce spermatogenesis after transplantation into the testes of an adult mouse azoospermia model
Zohreh Makoolati;
Zohreh Makoolati
1Department of Anatomical Sciences, Faculty of Medicine, Fasa University of Medical Sciences, Fasa, Iran
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Mansoureh Movahedin;
2Department of Anatomical Sciences, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran
Correspondence: Mansoureh Movahedin ([email protected])
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Mehdi Forouzandeh-Moghadam;
Mehdi Forouzandeh-Moghadam
3Department of Biotechnology, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran
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Majid Naghdi;
Majid Naghdi
1Department of Anatomical Sciences, Faculty of Medicine, Fasa University of Medical Sciences, Fasa, Iran
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Morteza Koruji
Morteza Koruji
4Cellular and Molecular Research Centre and Department of Anatomical Sciences, Iran University of Medical Sciences (IUMS), Tehran, Iran
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Publisher: Portland Press Ltd
Received:
May 31 2017
Revision Received:
July 12 2017
Accepted:
August 02 2017
Accepted Manuscript online:
August 02 2017
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© 2017 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society
2017
Clin Sci (Lond) (2017) 131 (18): 2381–2395.
Article history
Received:
May 31 2017
Revision Received:
July 12 2017
Accepted:
August 02 2017
Accepted Manuscript online:
August 02 2017
Citation
Zohreh Makoolati, Mansoureh Movahedin, Mehdi Forouzandeh-Moghadam, Majid Naghdi, Morteza Koruji; Embryonic stem cell derived germ cells induce spermatogenesis after transplantation into the testes of an adult mouse azoospermia model. Clin Sci (Lond) 15 September 2017; 131 (18): 2381–2395. doi: https://doi.org/10.1042/CS20171074
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