Although there are no conventional lymphatic vessels in the brain, fluid and solutes drain along basement membranes (BMs) of cerebral capillaries and arteries towards the subarachnoid space and cervical lymph nodes. Convective influx/glymphatic entry of the cerebrospinal fluid (CSF) into the brain parenchyma occurs along the pial-glial BMs of arteries. This project tested the hypotheses that pial-glial BM of arteries are thicker in the midbrain, allowing more glymphatic entry of CSF. The in vivo MRI and PET images were obtained from a 4.2-year-old dog, whereas the post-mortem electron microscopy was performed in a 12-year-old dog. We demonstrated a significant increase in the thickness of the pial-glial BM in the midbrain compared with the same BM in different regions of the brain and an increase in the convective influx of fluid from the subarachnoid space. These results are highly significant for the intrathecal drug delivery into the brain, indicating that the midbrain is better equipped for convective influx/glymphatic entry of the CSF.
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November 2017
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An atherosclerotic plaque. In Clinical Science volume 131, issue 21, Martín-Núñez et al. report on the relationship between Klotho, an anti-aging factor expressed in arterial walls, and inflammation in human atherosclerotic disease. For details, see pages 2601–2609.
Research Article|
November 13 2017
The perivascular pathways for influx of cerebrospinal fluid are most efficient in the midbrain
Howard Dobson;
Howard Dobson
1inviCRO, Boston, U.S.A.
2Department of Clinical Studies, University of Guelph, Ontario, Canada
3Department of Biomedical Physics, Western University
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Matthew MacGregor Sharp;
Matthew MacGregor Sharp
4Faculty of Medicine, Institute for Life Sciences, Southampton General Hospital, University of Southampton, South Academic Block, MP806, Tremona Road, Southampton, Hampshire SO166YD, U.K.
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Richard Cumpsty;
Richard Cumpsty
4Faculty of Medicine, Institute for Life Sciences, Southampton General Hospital, University of Southampton, South Academic Block, MP806, Tremona Road, Southampton, Hampshire SO166YD, U.K.
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Theodore P. Criswell;
Theodore P. Criswell
4Faculty of Medicine, Institute for Life Sciences, Southampton General Hospital, University of Southampton, South Academic Block, MP806, Tremona Road, Southampton, Hampshire SO166YD, U.K.
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Jenna M. Sullivan;
Jenna M. Sullivan
1inviCRO, Boston, U.S.A.
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Roy O. Weller;
Roy O. Weller
4Faculty of Medicine, Institute for Life Sciences, Southampton General Hospital, University of Southampton, South Academic Block, MP806, Tremona Road, Southampton, Hampshire SO166YD, U.K.
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Roxana O. Carare
4Faculty of Medicine, Institute for Life Sciences, Southampton General Hospital, University of Southampton, South Academic Block, MP806, Tremona Road, Southampton, Hampshire SO166YD, U.K.
Correspondence: Roxana O. Carare ([email protected])
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Publisher: Portland Press Ltd
Received:
August 04 2017
Revision Received:
October 07 2017
Accepted:
October 09 2017
Accepted Manuscript online:
October 11 2017
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© 2017 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society
2017
Clin Sci (Lond) (2017) 131 (22): 2745–2752.
Article history
Received:
August 04 2017
Revision Received:
October 07 2017
Accepted:
October 09 2017
Accepted Manuscript online:
October 11 2017
Citation
Howard Dobson, Matthew MacGregor Sharp, Richard Cumpsty, Theodore P. Criswell, Tyler Wellman, Ciara Finucane, Jenna M. Sullivan, Roy O. Weller, Ajay Verma, Roxana O. Carare; The perivascular pathways for influx of cerebrospinal fluid are most efficient in the midbrain. Clin Sci (Lond) 15 November 2017; 131 (22): 2745–2752. doi: https://doi.org/10.1042/CS20171265
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