Regeneration of ethanol-injured rat gastric mucosa must undergo changes in major metabolic pathways to achieve DNA replication and cell proliferation. These events are highly dependent on glucose utilization and inhibited by vitamin E (VE) (α-tocopherol) administration. Therefore, the present study aimed at assessing lipid metabolism in the gastric mucosa and ethanol-induced gastric damage and the effect of α-tocopherol administration. For this, rates of fatty acid β-oxidation and lipogenesis were tested in gastric mucosa samples. Through histological analysis, we found loss of the mucosa’s superficial epithelium, which became gradually normalized during the recovery period. Proliferation of gastric mucosa occurred with augmented formation of β-oxidation by-products, diminished synthesis of triacylglycerols (TGs), as well as of phospholipids, and a reduced cytoplasmic NAD/NADH ratio, whereas the mitochondrial redox NAD/NADH ratio was much less affected. In addition, α-tocopherol increased palmitic acid utilization in the gastric mucosa, which was accompanied by the induction of ‘mirror image’ effects on the cell redox state, reflected in an inhibited cell gastric mucosa proliferation by the vitamin administration. In conclusion, the present study shows, for the first time, the role of lipid metabolism in the adaptive cell gastric mucosa changes that drive proliferation after a chronic insult. Moreover, α-tocopherol increased gastric mucosa utilization of palmitic acid associated with energy production. These events could be associated with its antioxidant properties in co-ordination with regulation of genes and cell pathways, including changes in the cell NAD/NADH redox state.
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Double immunofluorescence staining for mindin (red) and CD68 (green) in atherosclerotic plaques of HFD-treated APOE-/- mice. In Issue 11 of Clinical Science, Zhu et al. discuss mindin deficiency as a novel mediator in protecting against foam cell formation and atherosclerosis, through direct interaction with LXR-β; for details, see pages 1199–1213.
Research Article|
June 26 2018
High α-tocopherol dosing increases lipid metabolism by changing redox state in damaged rat gastric mucosa and liver after ethanol treatment
Marisela Olguín-Martínez;
Marisela Olguín-Martínez
1Departamento de Biología Celular y Desarrollo, y de, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México (UNAM), Apartado Postal 70-243, Ciudad de México 04510, Mexico
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Diego R. Hernández-Espinosa;
Diego R. Hernández-Espinosa
2Departamento de Neurodesarrollo y Fisiología, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México (UNAM), Apartado Postal 70-243, Ciudad de México 04510, Mexico
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Rolando Hernández-Muñoz
1Departamento de Biología Celular y Desarrollo, y de, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México (UNAM), Apartado Postal 70-243, Ciudad de México 04510, Mexico
Correspondence: Rolando Hernández-Muñoz ([email protected])
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Publisher: Portland Press Ltd
Received:
February 12 2018
Revision Received:
May 14 2018
Accepted:
May 17 2018
Accepted Manuscript online:
May 17 2018
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© 2018 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society
2018
Clin Sci (Lond) (2018) 132 (12): 1281–1296.
Article history
Received:
February 12 2018
Revision Received:
May 14 2018
Accepted:
May 17 2018
Accepted Manuscript online:
May 17 2018
Citation
Marisela Olguín-Martínez, Diego R. Hernández-Espinosa, Rolando Hernández-Muñoz; High α-tocopherol dosing increases lipid metabolism by changing redox state in damaged rat gastric mucosa and liver after ethanol treatment. Clin Sci (Lond) 29 June 2018; 132 (12): 1281–1296. doi: https://doi.org/10.1042/CS20180154
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