The relationship between gut dysbiosis and obesity is currently acknowledged to be a health topic which causes low-grade systemic inflammation and insulin resistance and may damage the kidney. Organic anion transporter 3 (Oat3) has been shown as a transporter responsible for renal handling of gut microbiota products which are involved in the progression of metabolic disorder. The present study investigated the effect of probiotic supplementation on kidney function, renal Oat3 function, inflammation, endoplasmic reticulum (ER) stress, and apoptosis in obese, insulin-resistant rats. After 12 weeks of being provided with either a normal or a high-fat diet (HF), rats were divided into normal diet (ND); ND treated with probiotics (NDL); HF; and HF treated with probiotic (HFL). Lactobacillus paracasei HII01 1 × 108 colony forming unit (CFU)/ml was administered to the rats daily by oral gavage for 12 weeks. Obese rats showed significant increases in serum lipopolysaccharide (LPS), plasma lipid profiles, and insulin resistance. Renal Oat 3 function was decreased along with kidney dysfunction in HF-fed rats. Obese rats also demonstrated the increases in inflammation, ER stress, apoptosis, and gluconeogenesis in the kidneys. These alterations were improved by Lactobacillus paracasei HII01 treatment. In conclusion, probiotic supplementation alleviated kidney inflammation, ER stress, and apoptosis, leading to improved kidney function and renal Oat3 function in obese rats. These benefits involve the attenuation of hyperlipidemia, systemic inflammation, and insulin resistance. The present study also suggested the idea of remote sensing and signaling system between gut and kidney by which probiotic might facilitate renal handling of gut microbiota products through the improvement of Oat3 function.
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July 2018
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The 3D structure of recombinant human deoxyribonuclease I. The N-terminal leucine shown in yellow at the top of the structure was selected for PEGylation of the protein. The amino acids shown in yellow towards the bottom of the protein are those interacting with globular actin, a potent inhibitor of human deoxyribonuclease I; for details, see pages 1439–1452.
Research Article|
July 31 2018
Probiotic Lactobacillus paracasei HII01 protects rats against obese-insulin resistance-induced kidney injury and impaired renal organic anion transporter 3 function
Keerati Wanchai;
Keerati Wanchai
1Department of Physiology, Faculty of Medicine, Chiang Mai University, Thailand
7School of Medicine, Mae Fah Luang University, Chiang Rai, Thailand
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Sakawdaurn Yasom;
Sakawdaurn Yasom
2Department of Microbiology, Faculty of Medicine, Chiang Mai University, Thailand
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Wannipa Tunapong;
Wannipa Tunapong
1Department of Physiology, Faculty of Medicine, Chiang Mai University, Thailand
3Cardiac Electrophysiology Research and Training Center, Faculty of Medicine, Chiang Mai University, Thailand
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Titikorn Chunchai;
Titikorn Chunchai
1Department of Physiology, Faculty of Medicine, Chiang Mai University, Thailand
3Cardiac Electrophysiology Research and Training Center, Faculty of Medicine, Chiang Mai University, Thailand
8Center of Excellence in Cardiac Electrophysiology Research, Chiang Mai University, Chiang Mai, Thailand
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Sathima Eaimworawuthikul;
Sathima Eaimworawuthikul
3Cardiac Electrophysiology Research and Training Center, Faculty of Medicine, Chiang Mai University, Thailand
5Department of Oral Biology and Diagnostic Sciences, Faculty of Dentistry, Chiang Mai University, Thailand
8Center of Excellence in Cardiac Electrophysiology Research, Chiang Mai University, Chiang Mai, Thailand
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Parameth Thiennimitr;
Parameth Thiennimitr
2Department of Microbiology, Faculty of Medicine, Chiang Mai University, Thailand
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Chaiyavat Chaiyasut;
Chaiyavat Chaiyasut
4Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Thailand
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Anchalee Pongchaidecha;
Anchalee Pongchaidecha
1Department of Physiology, Faculty of Medicine, Chiang Mai University, Thailand
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Varanuj Chatsudthipong;
Varanuj Chatsudthipong
6Department of Physiology, Faculty of Science, Mahidol University, Bangkok, Thailand
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Siriporn Chattipakorn;
Siriporn Chattipakorn
3Cardiac Electrophysiology Research and Training Center, Faculty of Medicine, Chiang Mai University, Thailand
5Department of Oral Biology and Diagnostic Sciences, Faculty of Dentistry, Chiang Mai University, Thailand
8Center of Excellence in Cardiac Electrophysiology Research, Chiang Mai University, Chiang Mai, Thailand
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Nipon Chattipakorn;
Nipon Chattipakorn
1Department of Physiology, Faculty of Medicine, Chiang Mai University, Thailand
3Cardiac Electrophysiology Research and Training Center, Faculty of Medicine, Chiang Mai University, Thailand
8Center of Excellence in Cardiac Electrophysiology Research, Chiang Mai University, Chiang Mai, Thailand
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Anusorn Lungkaphin
1Department of Physiology, Faculty of Medicine, Chiang Mai University, Thailand
9Center for Research and Development of Natural Products for Health, Chiang Mai University, Thailand
Correspondence: Anusorn Lungkaphin ([email protected]; [email protected])
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Publisher: Portland Press Ltd
Received:
February 10 2018
Revision Received:
July 04 2018
Accepted:
July 05 2018
Accepted Manuscript online:
July 06 2018
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© 2018 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society
2018
Clin Sci (Lond) (2018) 132 (14): 1545–1563.
Article history
Received:
February 10 2018
Revision Received:
July 04 2018
Accepted:
July 05 2018
Accepted Manuscript online:
July 06 2018
Citation
Keerati Wanchai, Sakawdaurn Yasom, Wannipa Tunapong, Titikorn Chunchai, Sathima Eaimworawuthikul, Parameth Thiennimitr, Chaiyavat Chaiyasut, Anchalee Pongchaidecha, Varanuj Chatsudthipong, Siriporn Chattipakorn, Nipon Chattipakorn, Anusorn Lungkaphin; Probiotic Lactobacillus paracasei HII01 protects rats against obese-insulin resistance-induced kidney injury and impaired renal organic anion transporter 3 function. Clin Sci (Lond) 31 July 2018; 132 (14): 1545–1563. doi: https://doi.org/10.1042/CS20180148
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