Long noncoding RNAs (lncRNAs) have been highlighted to be involved in the pathological process of ischemic stroke (IS). The purpose of the present study was to investigate the expression profile of lncRNAs in peripheral blood mononuclear cells (PBMCs) of acute IS patients and to explore their utility as biomarkers of IS. Distinctive expression patterns of PBMC lncRNAs were identified by an lncRNA microarray and individual quantitative real-time PCR (qRT-PCR) in four independent sets for 206 IS, 179 healthy controls (HCs), and 55 patients with transient ischemic attack (TIA). A biomarker panel (lncRNA-based combination index) was established using logistic regression. LncRNA microarray analysis showed 70 up-regulated and 128 down-regulated lncRNAs in IS patients. Individual qRT-PCR validation demonstrated that three lncRNAs (linc-DHFRL1-4, SNHG15, and linc-FAM98A-3) were significantly up-regulated in IS patients compared with HCs and TIA patients. Longitudinal analysis of lncRNA expression up to 90 days after IS showed that linc-FAM98A-3 normalized to control levels by day 7, while SNHG15 remained increased, indicating the ability of lncRNAs to monitor IS dynamics. Receiver-operating characteristic (ROC) curve analysis showed that the lncRNA-based combination index outperformed serum brain-derived neurotrophic factor (BDNF) and neurone-specific enolase (NSE) in distinguishing IS patients from TIA patients and HCs with areas under ROC curve of more than 0.84. Furthermore, the combination index increased significantly after treatment and was correlated with neurological deficit severity of IS. The panel of these altered lncRNAs was associated with acute IS and could serve as a novel diagnostic method.
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The 3D structure of recombinant human deoxyribonuclease I. The N-terminal leucine shown in yellow at the top of the structure was selected for PEGylation of the protein. The amino acids shown in yellow towards the bottom of the protein are those interacting with globular actin, a potent inhibitor of human deoxyribonuclease I; for details, see pages 1439–1452.
Research Article|
July 31 2018
Differential long noncoding RNA expressions in peripheral blood mononuclear cells for detection of acute ischemic stroke
Qi-Wen Deng;
Qi-Wen Deng
*
1Department of Neurology, Affiliated ZhongDa Hospital, School of Medicine, Southeast University, Nanjing 210009, China
2Department of Neurology, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China
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Shuo Li;
Shuo Li
*
1Department of Neurology, Affiliated ZhongDa Hospital, School of Medicine, Southeast University, Nanjing 210009, China
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Huan Wang;
Huan Wang
1Department of Neurology, Affiliated ZhongDa Hospital, School of Medicine, Southeast University, Nanjing 210009, China
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Hui-Ling Sun;
Hui-Ling Sun
3General Clinical Research Center, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China
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Lei Zuo;
Lei Zuo
1Department of Neurology, Affiliated ZhongDa Hospital, School of Medicine, Southeast University, Nanjing 210009, China
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Zheng-Tian Gu;
Zheng-Tian Gu
1Department of Neurology, Affiliated ZhongDa Hospital, School of Medicine, Southeast University, Nanjing 210009, China
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Guo Lu;
Guo Lu
4Department of Neurology, Dezhou People’s Hospital, Dezhou 253014, China
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Cai-Zhi Sun;
Cai-Zhi Sun
5Department of Emergency, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China
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Han-Qing Zhang;
Han-Qing Zhang
1Department of Neurology, Affiliated ZhongDa Hospital, School of Medicine, Southeast University, Nanjing 210009, China
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Fu-Ling Yan
1Department of Neurology, Affiliated ZhongDa Hospital, School of Medicine, Southeast University, Nanjing 210009, China
Correspondence: Fu-Ling Yan ([email protected])
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Publisher: Portland Press Ltd
Received:
May 15 2018
Revision Received:
July 02 2018
Accepted:
July 09 2018
Accepted Manuscript online:
July 11 2018
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© 2018 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society
2018
Clin Sci (Lond) (2018) 132 (14): 1597–1614.
Article history
Received:
May 15 2018
Revision Received:
July 02 2018
Accepted:
July 09 2018
Accepted Manuscript online:
July 11 2018
Citation
Qi-Wen Deng, Shuo Li, Huan Wang, Hui-Ling Sun, Lei Zuo, Zheng-Tian Gu, Guo Lu, Cai-Zhi Sun, Han-Qing Zhang, Fu-Ling Yan; Differential long noncoding RNA expressions in peripheral blood mononuclear cells for detection of acute ischemic stroke. Clin Sci (Lond) 31 July 2018; 132 (14): 1597–1614. doi: https://doi.org/10.1042/CS20180411
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