Early recognition of vulnerable carotid plaques could help in identifying patients at high stroke risk, who may benefit from earlier revascularisation. Nowadays, different biomarkers of plaque instability have been unravelled, among these miRNAs are promising tools for the diagnosis and treatment of atherosclerosis. Inflammation, reactive oxygen species (ROS) and endothelial dysfunction play a key role in unstable plaques genesis. We showed that miR-200c induces endothelial dysfunction, ROS production and a positive mechanism among miR-200c and miR-33a/b, two miRNAs involved in atherosclerosis progression. The goal of the present study was to determine whether miR-200c could be an atherosclerosis biomarker. Carotid plaques of patients that underwent carotid endarterectomy (CEA) were assayed for miR-200c expression. miR-200c was up-regulated in carotid plaques (n=22) and its expression was higher in unstable (n=12) compared with stable (n=10) plaques. miR-200c positively correlated with instability biomarkers (i.e. monocyte chemoattractant protein-1, cicloxigenase-2 (COX2), interleukin 6 (IL6), metalloproteinase (MMP) 1 (MMP1), 9 (MMP9)) and miR-33a/b. Moreover, miR-200c negatively correlated with stability biomarkers (i.e. zinc finger E-box binding homoeobox 1 (ZEB1), endothelial nitric oxide (NO) synthase (eNOS), forkhead boxO1 (FOXO1) and Sirtuin1 (SIRT1)) (stable plaques = 15, unstable plaques = 15). Circulating miR-200c was up-regulated before CEA in 24 patients, correlated with miR-33a/b and decreased 1 day after CEA. Interestingly, 1 month after CEA, circulating miR-200c is low in patients with stable plaques (n=11) and increased to control levels, in patients with unstable plaques (n=13). Further studies are needed to establish whether miR-200c represents a circulating biomarker of plaque instability. Our results show that miR-200c is an atherosclerotic plaque progression biomarker and suggest that it may be clinically useful to identify patients at high embolic risk.
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α-Smooth muscle actin staining of perinatal nicotine exposed bone marrow mesenchymal stem cells (BMSCs) upon myogenic induction. In Clinical Science volume 132, issue 21, Sakurai et al. report that perinatal nicotine-induced BMSC myofibroblast differentiation can be prevented by augmenting the lipofibroblast phenotype; for details, see pages 2357–2368.
Research Article|
November 22 2018
Atherosclerotic plaque instability in carotid arteries: miR-200c as a promising biomarker
Alessandra Magenta
;
Alessandra Magenta
*
1Istituto Dermopatico dell’Immacolata, IDI-IRCCS, Via dei Monti di Creta 104, Rome 00167, Italy
Correspondence: Alessandra Magenta (ale.magenta@gmail.com)
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Sara Sileno;
Sara Sileno
*
1Istituto Dermopatico dell’Immacolata, IDI-IRCCS, Via dei Monti di Creta 104, Rome 00167, Italy
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Marco D’Agostino;
Marco D’Agostino
1Istituto Dermopatico dell’Immacolata, IDI-IRCCS, Via dei Monti di Creta 104, Rome 00167, Italy
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Francesca Persiani;
Francesca Persiani
2Department of Vascular Surgery, Istituto Dermopatico dell’Immacolata, IDI - IRCCS, Via dei Monti di Creta 104, Rome, Italy
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Sara Beji;
Sara Beji
1Istituto Dermopatico dell’Immacolata, IDI-IRCCS, Via dei Monti di Creta 104, Rome 00167, Italy
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Alessandra Paolini;
Alessandra Paolini
2Department of Vascular Surgery, Istituto Dermopatico dell’Immacolata, IDI - IRCCS, Via dei Monti di Creta 104, Rome, Italy
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Daniele Camilli;
Daniele Camilli
2Department of Vascular Surgery, Istituto Dermopatico dell’Immacolata, IDI - IRCCS, Via dei Monti di Creta 104, Rome, Italy
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Alessandro Platone;
Alessandro Platone
2Department of Vascular Surgery, Istituto Dermopatico dell’Immacolata, IDI - IRCCS, Via dei Monti di Creta 104, Rome, Italy
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Maurizio C. Capogrossi;
Maurizio C. Capogrossi
3Division of Cardiology, Johns Hopkins Bayview Medical Center, 4940 Eastern Avenue, Baltimore, MD 21224, U.S.A.
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Sergio Furgiuele
Sergio Furgiuele
2Department of Vascular Surgery, Istituto Dermopatico dell’Immacolata, IDI - IRCCS, Via dei Monti di Creta 104, Rome, Italy
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Clin Sci (Lond) (2018) 132 (22): 2423–2436.
Article history
Received:
August 02 2018
Revision Received:
October 31 2018
Accepted:
November 01 2018
Accepted Manuscript online:
November 02 2018
Citation
Alessandra Magenta, Sara Sileno, Marco D’Agostino, Francesca Persiani, Sara Beji, Alessandra Paolini, Daniele Camilli, Alessandro Platone, Maurizio C. Capogrossi, Sergio Furgiuele; Atherosclerotic plaque instability in carotid arteries: miR-200c as a promising biomarker. Clin Sci (Lond) 30 November 2018; 132 (22): 2423–2436. doi: https://doi.org/10.1042/CS20180684
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