1. A radioimmunoassay for angiotensin I is described.

2. An extraction procedure for angiotensin I from blood was developed, using alcoholic precipitation to stop the renin/renin substrate reaction.

3. The normal range found for angiotensin I in venous blood was 11–88 pg/ml and in arterial blood 12–72 pg/ml.

4. Angiotensin I levels fell following bilateral nephrectomy but angiotensin I was still detected several weeks following operation.

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