1. A rapid, robust, accurate and sensitive radioimmunoassay procedure is described for the measurement of plasma and urinary aldosterone.

2. A new, simple method is validated for the separation of free from bound moieties which has general applicability and which involves the measurement of free steroid by direct extraction into toluene-based liquid scintillator.

3. A new method, of validated specificity, is described for the measurement of the acid-labile metabolite of aldosterone in urine which avoids the necessity for preliminary chromatographic purification.

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