1. 1α,25-Dihydroxy-25-hemisuccinate cholecalciferol has been synthesized and conjugated to bovine serum albumin.

2. This conjugate is immunogenic; when injected into rabbits antibodies of high affinity for 1α,25-dihydroxycholecalciferol were obtained.

3. Vitamin D metabolites lacking the 1α-hydroxy group were of lower cross-reactivity with the antibodies.

4. By using these antibodies and 1α,25-[23,24-3H]dihydroxycholecalciferol as tracer a sensitive radioimmunoassay has been developed capable of detecting 20 pg of 1α,25-dihydroxycholecalciferol.

Keywords:
bovine serum albumin conjugate, cross-reactivity, 1α,25-dihydroxycholecalciferol, hemisuccinate, radioimmunoassay
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© 1978 The Biochemical Society and the Medical Research Society
1978
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