1. The location of α2-adrenoceptors has been examined in slide-mounted sections of dog kidney by using labelling in vitro with [3H]rauwolscine and autoradiography.

2. [3H]Rauwolscine binding in dog kidney was of high affinity, saturable, and to a single population of sites. Stereoselectivity was observed for (−)-noradrenaline and the relative affinity of a series of competing antagonists indicated binding to α2-adrenoceptors.

3. Autoradiography of [3H]rauwolscine binding revealed localization to three areas: glomeruli, the intimal and adventitial surfaces of arcuate blood vessels and the medullary vascular bundles.

Keywords:
α2-adrenoceptors, autoradiography, kidney, radioligand binding, [3H]rauwolscine
This content is only available as a PDF.
© 1985 The Biochemical Society and the Medical Research Society
1985
You do not currently have access to this content.