Interleukin-1β (IL-1β) increases the production of complement component C3 in enterocytes. Heat shock regulates the response to cytokines and other inflammatory mediators in various cell types. We tested the hypothesis that the heat-shock response regulates IL-1β-induced C3 production in the enterocyte. Cultured Caco-2 cells, a human intestinal epithelial cell line, were treated with sodium arsenite (10–500 µM) for 1 h or subjected to hyperthermia (43 °C) for 1–4 h, and allowed to recover for 1 h. The cells were then treated with IL-1β (0.5 ng/ml) for up to 24 h, whereafter C3 levels were measured by ELISA and C3 mRNA by Northern blot analysis. Heat-shock protein of 72 kDa (hsp72) was determined by Western blot analysis. Treatment of the cells with sodium arsenite or subjecting them to hyperthermia induced the expression of hsp72. The IL-1β-induced expression of C3 mRNA and C3 production were down-regulated by hyperthermia and sodium arsenite in a dose-dependent fashion. The results suggest that the stress response induced by hyperthermia or sodium arsenite decreases IL-1β-induced C3 production in human enterocytes.
Skip Nav Destination
Article navigation
Research Article|
July 21 1999
Stress response decreases the interleukin-1β-induced production of complement component C3 in human intestinal epithelial cells Available to Purchase
Ryan MOON;
Ryan MOON
*Department of Surgery, University of Cincinnati Medical Center, Cincinnati, OH 45267, U.S.A.
Search for other works by this author on:
Timothy A. PRITTS;
Timothy A. PRITTS
*Department of Surgery, University of Cincinnati Medical Center, Cincinnati, OH 45267, U.S.A.
Search for other works by this author on:
Alexander A. PARIKH;
Alexander A. PARIKH
†Shriners Burns Institute, Cincinnati, OH 45229, U.S.A.
Search for other works by this author on:
Josef E. FISCHER;
Josef E. FISCHER
*Department of Surgery, University of Cincinnati Medical Center, Cincinnati, OH 45267, U.S.A.
Search for other works by this author on:
Andrew L. SALZMAN;
Andrew L. SALZMAN
‡Division of Critical Care, Children's Hospital Medical Center, Cincinnati, OH 45229, U.S.A.
Search for other works by this author on:
Marnie RYAN;
Marnie RYAN
‡Division of Critical Care, Children's Hospital Medical Center, Cincinnati, OH 45229, U.S.A.
Search for other works by this author on:
Hector R. WONG;
Hector R. WONG
‡Division of Critical Care, Children's Hospital Medical Center, Cincinnati, OH 45229, U.S.A.
Search for other works by this author on:
Per-Olof HASSELGREN
*Department of Surgery, University of Cincinnati Medical Center, Cincinnati, OH 45267, U.S.A.
§Veterans Affairs Medical Center, Cincinnati, OH 45220, U.S.A.
Correspondence: Dr Per-Olof Hasselgren.
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
January 04 1999
Revision Received:
April 14 1999
Accepted:
June 01 1999
Online ISSN: 1470-8736
Print ISSN: 0143-5221
The Biochemical Society and the Medical Research Society © 1999
1999
Clin Sci (Lond) (1999) 97 (3): 331–337.
Article history
Received:
January 04 1999
Revision Received:
April 14 1999
Accepted:
June 01 1999
Citation
Ryan MOON, Timothy A. PRITTS, Alexander A. PARIKH, Josef E. FISCHER, Andrew L. SALZMAN, Marnie RYAN, Hector R. WONG, Per-Olof HASSELGREN; Stress response decreases the interleukin-1β-induced production of complement component C3 in human intestinal epithelial cells. Clin Sci (Lond) 1 September 1999; 97 (3): 331–337. doi: https://doi.org/10.1042/cs0970331
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Biochemical Society Member Sign in
Sign InSign in via your Institution
Sign in via your InstitutionGet Access To This Article
Cited By
Open Access for all
We offer compliant routes for all authors from 2025. With library support, there will be no author nor reader charges in 5 journals. Check here |
![]() |