β2-Microglobulin, a 12 kDa protein forming part of the class I HLA (histocompatibility locus antigen) major histocompatibility complex, has been used as a prognosis factor for multiple myeloma and as a marker of renal function, and has been shown to be involved in the pathogenesis of dialysis-related amyloidosis. α2-Macroglobulin has the ability to bind a wide range of physiologically important molecules, thereby influencing their metabolic impact. In this study we show by Western blotting analysis that β2-microglobulin binds to α2-macroglobulin in vitro. This binding was confirmed by BIAcore analysis, and was shown by ELISA to be concentration-dependent. The sequences of the binding peptides in the mature β2-microglobulin molecule were identified by Spot multiple peptide synthesis and α2-macroglobulin binding studies. In conclusion, β2-microglobulin interacts specifically with the universal antiprotease a2-macroglobulin. The identification of this interaction brings into question some of the axioms on the metabolism of β2-microglobulin, and may help to explain the clinical findings observed in b2-microglobulin-related diseases.
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March 24 2000
α2-Macroglobulin, the main serum antiprotease, binds β2-microglobulin, the light chain of the class I major histocompatibility complex, which is involved in human disease
Annie GOUIN-CHARNET;
Annie GOUIN-CHARNET
*Institut de Génétique Humaine (I.G.H.), CNRS UPR 1142, 141, rue de la Cardonille, 34090 Montpellier Cedex 5, France
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Daniel LAUNE;
Daniel LAUNE
†CNRS UMR 9921, University of Montpellier I, 34060 Montpellier Cedex 1, France
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Claude GRANIER;
Claude GRANIER
†CNRS UMR 9921, University of Montpellier I, 34060 Montpellier Cedex 1, France
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Jean-Claude MANI;
Jean-Claude MANI
†CNRS UMR 9921, University of Montpellier I, 34060 Montpellier Cedex 1, France
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Bernard PAU;
Bernard PAU
†CNRS UMR 9921, University of Montpellier I, 34060 Montpellier Cedex 1, France
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Georges MOURAD;
Georges MOURAD
‡Department of Nephrology, University Hospital Lapeyronie, 371, Av du Doyen Giraud, 34295 Montpellier Cedex, France
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Àngel ARGILÉS
*Institut de Génétique Humaine (I.G.H.), CNRS UPR 1142, 141, rue de la Cardonille, 34090 Montpellier Cedex 5, France
‡Department of Nephrology, University Hospital Lapeyronie, 371, Av du Doyen Giraud, 34295 Montpellier Cedex, France
Correspondence: Dr. A. ArgileéS, at Institut de Ge!ne!tique Humaine (e-mail [email protected]).
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Publisher: Portland Press Ltd
Received:
July 21 1999
Revision Received:
September 27 1999
Accepted:
December 01 1999
Online ISSN: 1470-8736
Print ISSN: 0143-5221
The Biochemical Society and the Medical Research Society © 2000
2000
Clin Sci (Lond) (2000) 98 (4): 427–433.
Article history
Received:
July 21 1999
Revision Received:
September 27 1999
Accepted:
December 01 1999
Citation
Annie GOUIN-CHARNET, Daniel LAUNE, Claude GRANIER, Jean-Claude MANI, Bernard PAU, Georges MOURAD, Àngel ARGILÉS; α2-Macroglobulin, the main serum antiprotease, binds β2-microglobulin, the light chain of the class I major histocompatibility complex, which is involved in human disease. Clin Sci (Lond) 1 April 2000; 98 (4): 427–433. doi: https://doi.org/10.1042/cs0980427
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