Quinolone antibiotics such as ciprofloxacin modify immune and inflammatory responses in some cells. We have shown previously that ciprofloxacin decreases the accumulation of interleukin (IL)-6 protein from a human endothelial cell line, whilst IL-8 protein production was increased. It is not known whether this occurs through effects on transcription and mRNA expression. We therefore investigated the effect of ciprofloxacin on mRNA for IL-6 and IL-8, and on three transcription factors known to be involved in the regulation of these cytokines. We investigated the effect of ciprofloxacin on tumour necrosis factor α- and IL-1β-mediated activation of the transcription factors nuclear factor κB (NFκB), activator protein-1 (AP-1) and nuclear factor IL-6 (NF-IL-6) using an electrophoretic mobility shift assay, and the effect on expression of mRNA for IL-6 and IL-8 by reverse transcriptase–PCR in the EAhy926 endothelial cell line. Ciprofloxacin decreased IL-6 mRNA (P < 0.05) and increased IL-8 mRNA (P < 0.05) expression. Ciprofloxacin did not modulate activation of NFκB or AP-1. However, NF-IL-6 binding was decreased in the presence of 100 µg/ml ciprofloxacin (P < 0.05). The study shows that ciprofloxacin-mediated decreased IL-6 release by a human endothelial cell line is reflected by decreased mRNA expression and decreased NF-IL-6 but not NFkB or AP-1 activation. Increased IL-8 mRNA in response to ciprofloxacin was not reflected by altered transcription factor activation and may represent increased mRNA stability.
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October 03 2000
Effect of ciprofloxacin on the activation of the transcription factors nuclear factor κB, activator protein-1 and nuclear factor-interleukin-6, and interleukin-6 and interleukin-8 mRNA expression in a human endothelial cell line
Helen F. GALLEY;
1Academic Unit of Anaesthesia & Intensive Care, University of Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen, AB 25 2ZD, Scotland
Correspondence: Dr H. F. Galley (e-mail [email protected]).
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Jatinder K. DHILLON;
Jatinder K. DHILLON
1Academic Unit of Anaesthesia & Intensive Care, University of Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen, AB 25 2ZD, Scotland
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Ross L. PATERSON;
Ross L. PATERSON
1Academic Unit of Anaesthesia & Intensive Care, University of Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen, AB 25 2ZD, Scotland
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Nigel R. WEBSTER
Nigel R. WEBSTER
1Academic Unit of Anaesthesia & Intensive Care, University of Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen, AB 25 2ZD, Scotland
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Publisher: Portland Press Ltd
Received:
March 15 2000
Revision Received:
June 14 2000
Accepted:
July 19 2000
Online ISSN: 1470-8736
Print ISSN: 0143-5221
The Biochemical Society and the Medical Research Society © 2000
2000
Clin Sci (Lond) (2000) 99 (5): 405–410.
Article history
Received:
March 15 2000
Revision Received:
June 14 2000
Accepted:
July 19 2000
Citation
Helen F. GALLEY, Jatinder K. DHILLON, Ross L. PATERSON, Nigel R. WEBSTER; Effect of ciprofloxacin on the activation of the transcription factors nuclear factor κB, activator protein-1 and nuclear factor-interleukin-6, and interleukin-6 and interleukin-8 mRNA expression in a human endothelial cell line. Clin Sci (Lond) 1 November 2000; 99 (5): 405–410. doi: https://doi.org/10.1042/cs0990405
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