Previous studies suggest that production of interleukin-6 (IL-6) is increased in the intestinal mucosa during sepsis and endotoxaemia. We tested the hypothesis that mucosal IL-6 production during endotoxaemia is increased further by the heat-shock (stress) response. The stress response was induced in mice by hyperthermia (rectal temperature of 42 °C for 3 min) or by intraperitoneal injection of sodium arsenite (10 mg/kg). At 2 h after induction of the stress response, groups of mice were injected subcutaneously with endotoxin (10 mg/kg) or sterile saline. IL-6 mRNA and protein levels in the jejunal mucosa were determined by an RNase protection assay and an ELISA respectively, and levels of hsp72 (heat-shock protein of 72 kDa) were determined by Western blot analysis. Hyperthermia and sodium arsenite increased hsp72 levels in the intestinal mucosa. IL-6 concentrations were increased in the jejunal mucosa of endotoxaemic mice, and this effect of endotoxaemia was potentiated by the stress response. Mucosal IL-6 mRNA levels were increased in endotoxaemic mice, and were increased further by the stress response. Thus it is concluded that mucosal IL-6 production during endotoxaemia may be further stimulated by the stress response. Increased IL-6 levels in the intestinal mucosa may be a potential mechanism by which the stress response exerts a protective effect during sepsis and endotoxaemia.
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November 07 2000
Induction of the stress response increases interleukin-6 production in the intestinal mucosa of endotoxaemic mice
Quan WANG;
Quan WANG
*Shriners Hospital for Children, Cincinnati, OH, U.S.A.
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Xiaoyan SUN;
Xiaoyan SUN
†Department of Surgery, University of Cincinnati, Cincinnati, OH, U.S.A.
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Timothy A. PRITTS;
Timothy A. PRITTS
†Department of Surgery, University of Cincinnati, Cincinnati, OH, U.S.A.
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Hector R. WONG;
Hector R. WONG
‡Children's Hospital Medical Center, Cincinnati, OH, U.S.A.
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Per-Olof HASSELGREN
†Department of Surgery, University of Cincinnati, Cincinnati, OH, U.S.A.
§Veterans Affairs Medical Center, Cincinnati, OH, U.S.A.
Correspondence: Dr Per-Olof Hasselgren, Department of Surgery, University of Cincinnati College of Medicine, 231 Bethesda Avenue, Cincinnati, OH 45267-0558, U.S.A. (e-mail [email protected].).
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Publisher: Portland Press Ltd
Received:
March 02 2000
Revision Received:
May 26 2000
Accepted:
August 03 2000
Online ISSN: 1470-8736
Print ISSN: 0143-5221
The Biochemical Society and the Medical Research Society © 2000
2000
Clin Sci (Lond) (2000) 99 (6): 489–496.
Article history
Received:
March 02 2000
Revision Received:
May 26 2000
Accepted:
August 03 2000
Citation
Quan WANG, Xiaoyan SUN, Timothy A. PRITTS, Hector R. WONG, Per-Olof HASSELGREN; Induction of the stress response increases interleukin-6 production in the intestinal mucosa of endotoxaemic mice. Clin Sci (Lond) 1 December 2000; 99 (6): 489–496. doi: https://doi.org/10.1042/cs0990489
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