HEK 293T cells were co-transfected with Flag-tagged JAG1 intracellular domain (Flag-JICD) and HA-tagged full-length MIB1 wild-type or mutants (HA-MIB1) (A, statistic assay of band density was calculated from two independent experiments) or Myc-tagged mib repeats domain region of MIB1 wild-type or variants (Myc-mib reps) (B). Twenty-four hours post-transfection, cell lysate was immunoprecipitated with Flag antibody immobilized beads, and samples were detected with respective antibodies.
HEK 293T cells were co-transfected with Flag-tagged JAG1 intracellular domain (Flag-JICD) and HA-tagged full-length MIB1 wild-type or mutants (HA-MIB1) (A, statistic assay of band density was calculated from two independent experiments) or Myc-tagged mib repeats domain region of MIB1 wild-type or variants (Myc-mib reps) (B). Twenty-four hours post-transfection, cell lysate was immunoprecipitated with Flag antibody immobilized beads, and samples were detected with respective antibodies.
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