The authors of article “MIB1 Mutations Reduce Notch Signaling Activation and Contribute to Congenital Heart Disease” (Clin Sci (2018) 132(23); https://doi.org/10.1042/CS20180732) had incorporated incorrect blots in the α-Flag panels of Figure 4B during their figure build. Below is a revised Figure 4 which includes the correct panels.

MIB1 mutations affect its interaction with JAG1

Figure 4
MIB1 mutations affect its interaction with JAG1

HEK 293T cells were co-transfected with Flag-tagged JAG1 intracellular domain (Flag-JICD) and HA-tagged full-length MIB1 wild-type or mutants (HA-MIB1) (A, statistic assay of band density was calculated from two independent experiments) or Myc-tagged mib repeats domain region of MIB1 wild-type or variants (Myc-mib reps) (B). Twenty-four hours post-transfection, cell lysate was immunoprecipitated with Flag antibody immobilized beads, and samples were detected with respective antibodies.

Figure 4
MIB1 mutations affect its interaction with JAG1

HEK 293T cells were co-transfected with Flag-tagged JAG1 intracellular domain (Flag-JICD) and HA-tagged full-length MIB1 wild-type or mutants (HA-MIB1) (A, statistic assay of band density was calculated from two independent experiments) or Myc-tagged mib repeats domain region of MIB1 wild-type or variants (Myc-mib reps) (B). Twenty-four hours post-transfection, cell lysate was immunoprecipitated with Flag antibody immobilized beads, and samples were detected with respective antibodies.