Issues

The cover of this issue of Clinical Science details ovarian cancer cells induced by ET-1 and their invasion of the stroma both in the areas adjacent to the central cancer mass and further into the stroma. Engineered ACMs consisting of 10% (w/v) collagen and HEY cells embedded within a stromal compartment of the same collagen density and stromal cells (HDF and HUVECs). Representative invasive bodies, fibroblasts, and endothelial structures adjacent to the invasive front of HEY biomimetic tumouroids. Drug treatment with ET-1 (100 nM) and/or macitentan (MAC) (1 μM). HEY cancer cell morphology was assessed using immunofluorescence of CK7 (red) and MMP14 (red) and DAPI (blue), HDF morphology using vimentin (green) and DAPI (blue), and HUVEC morphology using CD31 (green) and DAPI (blue). Scale bar = 100 μm. Read more in, ‘Endothelin-1 receptor blockade impairs invasion patterns in engineered 3D high-grade serous ovarian cancer tumoroids’ by Rosano’ and colleagues on pp 1441–1450.