Apolipoprotein E ( ApoE ) gene polymorphism is a major factor in lipid metabolism. It has been suggested that this polymorphism can modulate colorectal tumour risk. We tested this hypothesis for colorectal cancer (CRC). ApoE genotype was determined in 206 patients with CRC and 353 healthy controls from the East Anglia region of the U.K. Compared with individuals possessing the most common ɛ3/ɛ3 genotype, those with the ɛ2/ɛ3 genotype had an increased risk of colon cancer [odds ratio (OR) = 1.91; 95% confidence interval 1.05–3.45]. However, this association was strongly affected by gender. Separate analysis of male and female subjects revealed a highly significant association in men (OR = 2.71; 95% confidence interval 1.30–5.65), but no association in women (OR = 1.01; 95% confidence interval 0.37–2.77). Likewise, the proportion of male patients with more advanced tumours (Dukes' C&D) was significantly increased among those with the ApoE ε2/ε3 genotype (OR = 4.16; 95% confidence interval 1.36–12.75). No significant effect of the presence of the ε4 allele on CRC risk was found; however, there were no ε4/ε4 homozygotes among patients with proximal colon cancers. The ApoE ε3/ε3 majority genotype appeared to be associated with the lowest risk of CRC. These results suggest that ApoE genotype can influence both CRC risk and prognosis of the existing disease in a gender-dependent manner.

1. The oscillation method for measuring total respiratory resistance ( R rs ) is a simple method of assessing airway dimensions which can be applied in epidemiological surveys and potentially might be useful for detecting mild airway disease in smokers. However, it is not known whether abnormalities in R rs are only present when there are also abnormalities in simple spirometric tests. 2. We have compared values of R rs and its frequency-dependence ( f R ) using the oscillation technique applied over the frequency range 6-26 Hz in 42 healthy, non-asthmatic men who were never-smokers (aged 26-61 years) and in 41 male cigarette smokers (aged 32-64 years). The results were compared with those for spirometry and the single-breath N 2 test which are the most commonly used techniques in epidemiological surveys for detecting the effects of smoking on the lungs. 3. There was a strong trend for R rs (especially at lower oscillation frequency) and f R to increase with increasing age in smokers. Increases in R rs and f R were usually present when forced expiratory volume in 1 s was less than 80% of predicted and the forced expiratory volume in 1 s/vital capacity ratio was less than 65%, but abnormal f R was present in some smokers whose spirometry was within conventional normal limits. 4. Abnormalities in R rs and f R were weakly associated with abnormality of the single-breath N 2 manoeuvre. 5. Abnormal f R is normally attributed to uneven narrowing of intrathoracic airways; however, in smokers it was associated with an increase in R rs at 6 Hz, so we cannot exclude that some of the observed abnormal f R was due to increased dissipation of the applied pressure in the cheeks and extrathoracic airway rather than to in-homogeneities within the lungs. 6. We conclude that the oscillation technique detects abnormalities indicating airway narrowing in some smokers whose spirometry is within normal limits. Hence the technique could be useful in screening programmes aiming to detect early lung damage. The prognostic significance of the additional information provided by measuring R rs needs to be further assessed.

1. The safety of a carbon haemoperfusion column, Haemocol, has been studied in beagle dogs. 2. Only minor changes in physiology, haematology and plasma chemistry were detected, except those associated with an improvement in clinical status. 3. It is concluded that haemoperfusion through this column is a safe and simple procedure, which merits evaluation in the treatment of severe drug overdose in man.