... weight of fibroid explants which had been transduced by lentivirus for either control (empty vector) or MIAT knockdown for four weeks ( n =7). Knockdown of MIAT in fibroid xenografts resulted in a 30% reduction of tumor weight and a marked increase in miR-29a, -b, and -c levels in the xenografts...

... over 20% sucrose (22000 rpm, 2 h) then resuspended in PBS. The functional titer of each batch of lentivirus, in transforming units per ml, was quantified by transducing 293FT cells with serial dilutions of the viral suspension then determining the percentage of EGFP fluorescent cells using flow...

... in specific target cell populations is continually advancing. Gene therapy for the nervous system is particularly challenging due to the post-mitotic nature of neuronal cells and the restricted accessibility of the brain itself. Viral vectors based on lentiviruses provide particularly attractive vehicles...