... antibodies were of very low affinity and/or directed against non-subunit THP. 5. The pathological relevance of human anti-THP antibodies measured by ELISA remains to be established. © 1970 The Biochemical Society and the Medical Research Society 1970 affinity chromatography autoantibodies enzyme...

...Tamiko Ohsawa; Shigehisa Hirose; Tadashi Inagami; Kazuo Murakami 1. Renin was purified to homogeneity from bovine anterior pituitary by using batchwise DEAE-cellulose chromatography, pepstatin-aminohexyl-agarose affinity chromatography, Ultrogel AcA 44 gel filtration and DEAE-Sephacel and CM...

... Reference Preparation, should be used with caution or replaced by proteinase-free human renin which can be easily prepared by use of suitable affinity chromatography. 28 11 1980 22 12 1980 © 1981 The Biochemical Society and the Medical Research Society 1981 affinity chromatography...

...S. A. Atlas; J. H. Laragh; Jean E. Sealey; T. E. Hesson 1. Plasma prorenin (inactive renin), which accounts for about 70% of the total renin in human plasma, was almost completely separated from active renin by affinity chromatography on Cibacron blue F3G-A-agarose. The slight residual renin...

...V. J. Dzau; Amy Brenner; Neremiah Emmett; E. Haber 1. The major angiotensin I-generating activity of rat brain extracts has a pH optimum different from that of renal renin and is not inhibited by renin specific antibody. 2. Affinity chromatography utilizing renin specific antibody, pepstatin and α...

...Eve E. Slater; Robert C. Cohn; Victor J. Dzau; Edgar Haber 1. Human renal renin has been purified 200 000-fold from cadaver kidney cortex by a method which employs affinity chromatography on aminohexyl pepstatin. 2. The product of this purification has a specific activity of 400 Goldblatt units/mg...

...Tadashi Inagami; Hideyoshi Yokosawa; Shigehisa Hirose 1. Angiotensin I-generating activity of rat brain extract was separated into two components by affinity chromatography on a casein—Sepharose gel column. 2. The component without affinity to the gel was identified as true renin on the basis...

.... Immunoreactive labelled HPTH in the medium was extracted by affinity chromatography. 4. In cultures from glands, six adenomatous and one hyperplastic, radioactive HPTH in the media was indistinguishable in size and charge from highly purified bovine parathyroid hormone (PTH). 5. In two cultures from adenomata...