.... polyubiquitin, C2α proteasome subunit, 14kDa ubiquitin-carrier protein and ubiquitin-activating protein, and of two lysosomal proteolytic enzymes, i.e. cathepsin B and cathepsin D, were measured using quantitative Northern blotting. mRNA levels for cathepsin B, but not for components of the ubiquitin-proteasome...

... (cathepsin B, N -β-acetyl-galactosaminidase and N -acetyl-β-glucosaminidase) compared with basal values for 2 h after instillation. 4. In addition, there were significant correlations between cathepsin B secretion and amylase secretion ( r = 0.7764, P< 0.001) and between cathepsin B secretion and protein...

...David Burnett; Robert A. Stockley 1. Cathepsin B-like activity was measured in lung secretions by using the fluorimetric substrate benzyloxycarbonyl- l -arginine- l -arginine-4-methyl-7-coumarylamide (Z-Arg-Arg-MEC). The enzyme had a pH optimum of approximately 5.5 and had the characteristics...