.... homocysteine, AdoMet ( S -adenosylmethionine), AdoHcy ( S -adenosylhomocysteine), serine and glycine. Primed, continuous infusions with [ 2 H 3 -methyl-1- 13 C]methionine were used to determine flux rates of methionine transmethylation, homocysteine remethylation and homocysteine trans-sulphuration. Metabolic...

...Alan A. JACKSON; Rebecca L. DUNN; Michael C. MARCHAND; Simon C. LANGLEY-EVANS When rat dams consume a diet low in protein during pregnancy, their offspring develop high blood pressure. On a low-protein diet, the endogenous formation of the amino acid glycine is thought to become constrained...

... turnover in normal pregnant women. A group of 27 multiparous Caucasian women with singleton pregnancies were studied at around 18 and 28 weeks' gestation. Body composition was determined by anthropometry, and whole-body protein turnover was estimated by using a single oral dose of [ 15 N]glycine...

...G. E. A. Bettany; B. C. Ang; S. N. Georgiannos; D. Halliday; J. Powell-Tuck 1. Whole-body protein turnover was measured using the [ 15 N]glycine tracer technique in 10 post-absorptive healthy volunteers during normal daily activities and a period of bed rest. 2. Bed rest reduced whole-body protein...

...A. A. El Sayed; J. Haylor; A. M. El Nahas 1. The direct effects of individual amino acids, including glycine (a neutral amino acid), l-glutamic acid (an acidic amino acid), l-leucine (a neutral, branched-chain amino acid) and l-arginine (a basic amino acid), on renal function were compared...

... the urinary excretion of ammonia and total urea over a 12 h experimental period. 2. Except with oral glycine and alanine, the synthesis rates given by ammonia and urea were appreciably different within each study when calculated on the assumption of a single pool of metabolic nitrogen in the body. In general...

... of 15 N in ammonia and in urea over a 9 h period after an oral dose of [ 15 N]glycine. 2. The food intake during the 2 study days was identical for individual patients. The amount each received was determined from measurement of their intake of food ad libitum on the day preceding the pre-operative...

...E. B. Fern; P. J. Garlick; Margaret A. McNurlan; J. C. Waterlow 1. Four normal adults were given [ 15 N]-glycine in a single dose either orally or intravenously. Rates of whole-body protein turnover were estimated from the excretion of 15 N in ammonia and in urea during the following 9 h. The rate...

...M. Taruvinga; A. A. Jackson; M. H. N. Golden 1. Whole-body protein turnover was measured in rats by constant infusion of 15 N-labelled glycine, aspartate, valine and leucine and measuring the enrichment of hepatic and renal urea and ammonia nitrogen. 2. The values obtained with [ 15 N]glycine were...

...J. A. Nicholson; T. J. Peters 1. The subcellular distribution of peptidase activities in the normal human jejunum against glycine and leucine homopeptides has been investigated with an analytical fractionation technique. 2. An 8000 g -min supernatant was prepared from homogenates of Crosby capsule...

...M. H. N. Golden; J. C. Waterlow 1. Total body protein turnover was studied in six elderly patients. 2. During the study they were fed by continuous infusion of a liquid formula through a nasogastric tube. l-[1- 14 C]Leucine and [ 15 N]glycine were infused at a constant rate for 30 h. The labelled...

...R. C. Heading; H. P. Schedl; L. D. Stegink; D. L. Miller 1. Absorption of [ 3 H]glycine and [ 14 C]-glycyl-l-proline at concentrations between 0·5 and 4 mmol/l was studied by perfusion in vivo of rat jejunal and ileal segments. Absorption was defined as net removal of radioisotope from...

...T. J. Peters 1. The relative rates of hydrolysis of a series of glycine homopeptides by guinea-pig small intestinal mucosa and by isolated brush borders have been studied. Oligopeptides up to and including hexaglycine were hydrolysed. No activity was detected against a series of homopolypeptides...

...G. C. Cook 1. Using a double-lumen tube perfusion system the rates of glycine, glycylglycine, and glycylglycine and glucose absorption from a 30-cm jejunal segment have been studied in vivo in a group of relatively normal Zambian African subjects. 2. To determine the kinetic curve for glycine...

...G. C. Cook 1. To investigate the effect of different intraluminal concentrations on the mutual inhibitive effect of glycine and glucose on their jejunal absorption rates, eighteen convalescent Zambian African patients who had no clinical evidence of intestinal disease or of malnutrition were given...