... of ingested glucose as glycogen could be responsible during the post-meal suppression of plasma NEFAs (non-esterified fatty acids; ‘free’ fatty acids). In the present study we determined the metabolic basis of the second-meal phenomenon. Healthy subjects were studied on two separate days, with breakfast...

...Luc J. C. VAN LOON; Robyn MURPHY; Audrey M. OOSTERLAAR; David CAMERON-SMITH; Mark HARGREAVES; Anton J. M. WAGENMAKERS; Rodney SNOW It has been speculated that creatine supplementation affects muscle glucose metabolism in humans by increasing muscle glycogen storage and up-regulating GLUT-4 protein...

... of frozen cervical biopsies were split in two. Half of the material was precipitated by ethanol in the presence of glycogen, and in the remaining half glycogen was absent. 3. On average a 15-fold increase in total DNA yield was obtained with glycogen. As a result, in 63 cases analysed by PCR for human...

... reoxygenation than the remainder of the subjects (13 compared with 22 s, P = 0.004). 3. Thinness at birth did not influence muscle capillary density, muscle glycogen content, glycogen synthase activity, citrate synthase activity or resting forearm blood flow. 4. Insulin resistance seen after fetal malnutrition...

...R. F. G. J. King; M. Madan; D. Alexander; A. Boyd; K. Ibrahim; M. J. McMahon; D. Johnston 1. This study was designed to test the hypothesis that three-carbon intermediates can be used in the ‘indirect’ pathway of glycogen synthesis in human liver (i.e. a route additional to the use of glucose...

.... 3. Wedge biopsies of liver were obtained at laparotomy and analysed for glycogen concentration, the activity of three key enzymes of glucose metabolism, 6-phosphofructokinase (EC 2.7.1.11), fructose bisphosphatase (EC 3.1.3.11) and hexokinase (EC 2.7.1.1), and the capacity for protein synthesis. 4...

... rates were unaffected by starvation. By contrast, glycogen concentrations were decreased even after 8 post-operative days, by which time liver mass had been restored and concentrations were sensitive to nutritional status. 3. The results of experiments involving the intragastric or intraperitoneal...

... was measured and muscle biopsies were taken for analysis of fibre cross-sectional areas of type 1 and type 2 fibres as well as adenosine 5′-triphosphate, creatine phosphate and glycogen contents. 3. From the results it is concluded that passive muscle stretching leads to a significant increase in hip abduction...

... after 10 min maximal exercise (arm ergometer). The proportion of PDH in the active form increased least after immobilization (to 52 ± 13.6%), compared with control (95 ± 11.7%) and post-training (98 ± 6.6%). 4. After exercise muscle glycogen fell to the greatest extent and lactate rose the least...

...C. S. Harmon; P. J. R. Phizackerley 1. The observation that the glycogen content of epidermis from psoriatic lesions and from regenerating wound epithelium is increased has been confirmed by quantitative estimation. 2. In epidermis from psoriatic lesions, although the proportion of glycogen...

...F. C. Martin; A. J. Levi; G. Slavin; T. J. Peters 1. The capacity for glycolysis in muscle biopsies obtained from long-term heavy alcohol drinking patients has been compared with tissue from control subjects by assay in vitro of the total activities of glycogen phosphorylase, phosphofructokinase...

...G. M. Hall; Celine Traynor; J. L. Paterson 1. Changes in liver glycogen and glycolytic intermediates were determined in ten patients undergoing abdominal surgery for carcinoma of the colon. 2. One hour of surgery resulted in a 19% decrease in liver glycogen, together with an increase in hepatic...

... duration ( n = 3), only 39 ± 1% of the enzyme was in the active form. 6. Muscle glycogen depletion was greatest with intermittent exercise and least with isometric maximal exercise; in contrast, the increase in muscle lactate was least with progressive exercise (1·3 to 9·4 μmol/g), intermediate...

... with alkalosis to 17.1 ± 2.5 μmol/g and least with acidosis to 12.2 ± 1.4 μmol/g. Muscle glycogen depletion was comparable in control and alkalosis studies. 5. The lower plasma lactate concentration during exercise in acidosis compared with control and alkalosis appears to be due to an inhibition of muscle...

...T. J. Peters; W. Jenkins; V. Dubowitz 1. An homogenate of liver biopsy taken from a patient with Pompe's disease (type II glycogen-storage disease) was analysed by enzyme microassay and subcellular fractionation by sucrose-density-gradient centrifugation. 2. A strikingly low activity of lysosomal...

...G. Y. Ma; C. D. Gove; M. A. Cawthorne; D. A. Hems 1. Rapid effects of hormones on the metabolism of glycogen and fatty acids were studied in the perfused liver of normal and genetically obese ( ob / ob ) mice. 2. In livers from normal and obese mice adrenaline and angiotensin II stimulated...

...A. Juhlin-Dannfelt; L. Jorfeldt; L. Hagenfeldt; B. Hulten 1. The influence of ethanol administration on the metabolism of non-esterified fatty acids (NEFA) and on the utilization of blood-borne substrates and stored glycogen by leg muscles was examined in nine young healthy subjects at rest...

... concentration and no impairment in their hypoglycaemic response to exogenous insulin administration. Furthermore, fasting malnourished rats were unable to correct the insulin-induced hypoglycaemia despite high concentrations of hepatic glycogen. 5. Malnourished rats had lower peak plasma insulin concentrations...

... in the two groups. 3. ATP, creatine phosphate and glycogen content of muscle were significantly reduced in the basal state in the patients. During exercise, ATP fell markedly but the lactate content of muscle was within the normal range. Muscle glycogen utilization during exercise exceeded...