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Keywords: high-performance liquid chromatography
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Articles
Journal:
Clinical Science
Clin Sci (Lond) (1996) 91 (5): 633–638.
Published: 01 November 1996
... babies born prematurely. high-performance liquid chromatography non-transferrin-bound-iron preterm babies 19 7 1996 25 7 1996 © 1996 The Biochemical Society and the Medical Research Society 1996 Clinical Science (1996) 91,633-638 (Printed in Great Britain) 633 Chromatographic...
Abstract
1. Non-protein-bound iron has been implicated in the aetiology of chronic lung disease of prematurity. 2. The modification of a method for the measurement of non-transferrin-bound iron in small volumes of plasma and bronchoalveolar lavage fluid from preterm babies is described. 3. The assay runs with a good degree of precision and a lower limit of detection of 0.02 μmol/l. 4. Non-transferrin-bound iron was detected in 50% of plasma samples and 11% of bronchoalveolar lavage fluid samples collected over the first week of life from babies born prematurely.
Articles
Journal:
Clinical Science
Clin Sci (Lond) (1996) 90 (1): 37–46.
Published: 01 January 1996
... endothelin high-performance liquid chromatography human endocardial endothelial cells immunocytochemistry reverse transcriptase—polymerase chain reaction radioimmunoassay Clinical Science (1996) 90, 3 7 4 (Printed in Great Britain) 37 Expression of endothelin peptides and mRNA in the human heart C...
Abstract
1. We have examined the expression of endothelin isoforms and their precursors in the human heart using RIA, HPLC, immunocytochemistry and reverse transcriptase—polymerase chain reaction assays. 2. Highly specific RIAs were used to measure the levels of mature endothelin and big endothelin-1 immunoreactivity in extracts of human right ventricle. There was no significant difference between samples from patients with ischaemic heart disease and idiopathic dilated cardiomyopathy. 3. HPLC coupled with RIAs allowed the separation and identification of the three mature isoforms of endothelin, big endothelin-1 and the C-terminal fragment of big endothelin-1. In extracts of human endocardial endothelial cells, peaks of immunoreactivity that co-eluted with authentic endothelin-1, big endothelin-1 and C-terminal fragment were found. 4. Intense immunocytochemical staining of mature endothelin immunoreactivity was detected in the cytoplasm of endothelial cells of all regions of the heart tested. Big endothelin-1 immunoreactivity mirrored that of the mature peptide and, in two of three individuals tested, big endothelin-2 immunoreactivity was also detected. No big endothelin-3 immunoreactivity was detected in any of the tissues examined. 5. Reverse transcriptase—polymerase chain reaction assays demonstrated endothelin-1 and endothelin-2 mRNA in all three samples of human left ventricle tested. In two of the individuals, additional bands were also detected with the endothelin-2 primers which corresponded to splice variants. There was no evidence for the expression of endothelin-3 mRNA. 6. These data suggest that endothelin-1 is the predominant isoform of endothelin in the human heart and is probably largely synthesized by the endothelial cells within the heart. If released from the endothelial cells in vivo , this potent cardiotonic peptide may play an important paracrine role in human cardiovascular function.
Articles
Journal:
Clinical Science
Clin Sci (Lond) (1990) 78 (1): 63–66.
Published: 01 January 1990
... subjects, there was a marked deficit of menaquinone-8. 19 7 1989 27 7 1989 © 1990 The Biochemical Society and the Medical Research Society 1990 electrochemistry high-performance liquid chromatography menaquinones vitamin K Clinical Science (1990) 78,63-66 63 Age-related changes...
Abstract
1. Through the vitamin K 1 cycle, phylloquinone is now known to play an active role, not only in relation to prothrombin, but also in the synthesis of bone peptides. 2. The recent development of a sensitive method allowed the demonstration of a deficit of vitamin K 1 in the circulation of osteoporotic subjects. 3. Vitamin K 2 , namely the menaquinones of various chain-lengths, has been shown by others to be more effective than vitamin K 1 in the curative rat bioassay. 4. Earlier reports had shown that the concentration of menaquinones in human liver may exceed that of vitamin K 1 . But previous methods were too insensitive for testing the normal circulating levels of menaquinones in the human. 5. The new sensitive method has now been applied to measuring the circulating levels of vitamin K 1 and of two of the menaquinones, namely menaquinone-7 and menaquinone-8. 6. In normal individuals, the circulating levels of vitamin K 1 were the same, irrespective of age. 7. In young normal subjects, the combined levels of menaquinone-7 and menaquinone-8 were at least the same as the level of vitamin K 1 . In elderly normal subjects, there was a marked deficit of menaquinone-8.
Articles
Journal:
Clinical Science
Clin Sci (Lond) (1986) 71 (2): 211–215.
Published: 01 August 1986
...]dihydroxymetabolites by high-performance liquid chromatography (HPLC). 2. After a 30 min intravenous infusion, [ 3 H]NA accounted for 57.2 ± 13.2% of the radioactivity recovered by the procedure, while the dihydroxymetabolites 3,4-[ 3 H]dihydroxyphenylethylene glycol ([ 3 H]DHPG) and 3,4-[ 3 H]dihydroxymandelic acid...
Abstract
1. An improved approach for the determination of plasma [ 3 H]noradrenaline ([ 3 H]NA) kinetics in man is described, incorporating the extraction of plasma [ 3 H]catechols on alumina and separation of [ 3 H]NA from [ 3 H]dihydroxymetabolites by high-performance liquid chromatography (HPLC). 2. After a 30 min intravenous infusion, [ 3 H]NA accounted for 57.2 ± 13.2% of the radioactivity recovered by the procedure, while the dihydroxymetabolites 3,4-[ 3 H]dihydroxyphenylethylene glycol ([ 3 H]DHPG) and 3,4-[ 3 H]dihydroxymandelic acid ([ 3 H]DOMA) accounted for 32.3 ± 11.5% and 4.9 ± 6.0% respectively. After 90 min of constant infusion the proportion due to [ 3 H]NA fell to 44.4 ± 10.4%, while that due to [ 3 H]DHPG rose to 45.9 ± 9.5% because of an increase in the amount of [ 3 H]DHPG at the later time. 3. Plasma [ 3 H]NA radioactivity rose rapidly during the constant infusion and usually reached a plateau by 30 min. However, in individual subjects large variations in plasma [ 3 H]NA radioactivity occurred during the course of the infusion, implying rapid and variable changes in plasma [ 3 H]NA clearance. 4. The inclusion of a step to separate [ 3 H]NA from [ 3 H]dihydroxymetabolites is necessary if the aim is to determine plasma [ 3 H]NA kinetics, as a large proportion of the radioactivity recovered from plasma on alumina is due to the presence of these metabolites.
Articles
Journal:
Clinical Science
Clin Sci (Lond) (1985) 69 (1): 1–6.
Published: 01 July 1985
...C. C. T. Smith; L. D. Curtis; A. P. Delamothe; B. N. C. Prichard; D. J. Betteridge 1. We have used high-performance liquid chromatography with electrochemical detection to measure content of adrenaline and noradrenaline in platelets in 13 normal subjects at rest. 2. Subjects were exercised to raise...
Abstract
1. We have used high-performance liquid chromatography with electrochemical detection to measure content of adrenaline and noradrenaline in platelets in 13 normal subjects at rest. 2. Subjects were exercised to raise plasma catecholamine levels and promote the platelet release reaction. 3. There was a significant positive correlation between plasma noradrenaline concentrations and platelet noradrenaline content. 4. Platelet/plasma concentration ratios were 1855 for noradrenaline and 268 for adrenaline at rest and 473 and 152 respectively after exercise. 5. Plasma noradrenaline levels positively correlated with age. 6. Determination of platelet factors released to the plasma showed increases of β-thromboglobulin and platelet factor 4 with exercise, whereas thromboxane B 2 remained unchanged. No change in platelet catecholamine levels occurred with exercise and no correlations were observed between platelet catecholamines and released platelet factors. 7. These data suggest that plasma catecholamine levels influence platelet content and that noradrenaline and adrenaline are concentrated in platelets.
Articles
Journal:
Clinical Science
Clin Sci (Lond) (1982) 62 (2): 209–213.
Published: 01 February 1982
... excretion. 14 7 1981 20 7 1981 © 1982 The Biochemical Society and the Medical Research Society 1982 dopamine high-performance liquid chromatography indomethacin prostaglandins sodium excretion Clinical Science (1982) 62,209-213 209 Effect of inhibition of prostaglandin...
Abstract
1. The effect of inhibition of prostaglandin synthesis on urinary excretion of free dopamine, a catecholamine thought to act as a renal natriuretic hormone, was examined in six normal women. 2. The volunteer subjects were treated with indomethacin (2 mg day −1 kg −1 ) for 7 days. This regimen produced an immediate and significant but transient decrease in urinary sodium excretion, averaging about 80 mmol over a period of 3 days. 3. Urinary dopamine excretion during the control period was not different from that during the entire period of indomethacin treatment. 4. It is concluded that indomethacin does not affect urinary dopamine excretion. The change in intrarenal nervous release of dopamine in response to the sodium retention induced by indomethacin may have been too small to make a significant contribution to total urinary dopamine excretion.