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Keywords: leucocytes
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Articles
Clin Sci (Lond) (2015) 128 (10): 723–733.
Published: 10 March 2015
... in the vasculature. Cryopreservation of leucocytes is routinely used for long-term storage and determination of phenotypic/functional changes at a later date. Exploring the role of bioenergetics and mitochondrial (dys)function in leucocytes is often examined by using freshly isolated cells. The aim of the pilot...
Articles
Clin Sci (Lond) (2001) 100 (1): 1–12.
Published: 08 December 2000
...Michael J. HICKEY Constitutively produced nitric oxide released by endothelial cells has been shown to act as an endogenous agent which inhibits the rolling and adhesion of leucocytes in the microcirculation. However, during various types of inflammation, expression of the inducible form of nitric...
Articles
Clin Sci (Lond) (1999) 97 (5): 595–601.
Published: 28 September 1999
...Sunil Kumar CHHABRA; Anita KHANDUJA; Deepika JAIN The present study was carried out to determine the intracellular free calcium concentration ([Ca 2+ ] i ) and the activity of its regulatory enzymes (Na + ,K + -ATPase and Ca 2+ -ATPase) in leucocytes. Levels of plasma lysophosphatidylcholine (LPC...
Articles
Clin Sci (Lond) (1995) 88 (6): 695–700.
Published: 01 June 1995
... free [K + ]. 10 2 1995 23 2 1995 © 1995 The Biochemical Society and the Medical Research Society 1995 leucocytes lymphocytes Na + -K + -exchanging ATPase ouabain potassium Clinical Science (1995) 88, 695-700 (Printed in Great Britain) 695 Effect of extracellular potassium...
Articles
Clin Sci (Lond) (1991) 81 (3): 405–412.
Published: 01 September 1991
...M. Hogg; L. L. Ng; J. E. Davies 1. A novel triple-isotope method using 3 H 2 O, [ 14 C]-sucrose and 36 Cl − for measuring initial Cl − uptake rates in human leucocytes that had been preincubated in 10% (v/v) autologous serum is described. 2. There was a marked dependence of Cl − influx...
Articles
Clin Sci (Lond) (1991) 81 (2): 209–213.
Published: 01 August 1991
... examined in rats with 24 h bilateral ureteral obstruction. 3. Irradiation effectively prevented the leucocyte infiltrate after bilateral ureteral obstruction (1.2 ± 0.8 × 10 5 versus 27.1 ± 0.1 × 10 5 cells/g of cortex, n = A in each group), resulted in a significantly higher inulin clearance (2.78 ± 0.27...
Articles
Clin Sci (Lond) (1991) 80 (2): 95–99.
Published: 01 February 1991
...-sensitive fluorescent dye, bis(carboxyethyl)carboxyfluorescein. The change in external Na + concentrations for all pH measurements was similar in both cell types. 2. A significant difference between the two types of cells was found, the polymorphonuclear leucocytes having a higher Na + /H + antiport...
Articles
Clin Sci (Lond) (1990) 79 (3): 247–252.
Published: 01 September 1990
.... Polymorphonuclear cell zinc was decreased in both patient groups and correlated strongly with abdominal muscle zinc ( r = 0.89, P < 0.001). 4. Mononuclear cell and total leucocyte zinc were similar to control values in both groups of patients. Total leucocyte, but not mononuclear cell, zinc correlated weakly...
Articles
Clin Sci (Lond) (1990) 78 (3): 335–341.
Published: 01 March 1990
... proposed. We have developed a triple-isotope method for estimating myo -inositol influx into human leucocytes and so investigated both the kinetics of this uptake in normal volunteers and the effect of glucose upon it. 2. Uptake was composed of a passive component with a rate constant of 2.4 ± 0.3 × 10 −2...
Articles
Clin Sci (Lond) (1990) 78 (2): 235–238.
Published: 01 February 1990
...J. Main; T. Thomas 1. Although ethanol appears to alter cellular sodium transport, the exact nature of its effects has not been clearly defined. We have studied the effects of different concentrations of ethanol on human leucocyte sodium content, and on the rise in leucocyte sodium content which...
Articles
Clin Sci (Lond) (1989) 77 (4): 417–423.
Published: 01 October 1989
...L. L. Ng; C. Dudley 1. A modified method is described for holding the intracellular pH of leucocytes at different values for prolonged periods of time. 2. Using ammonium sulphate at a final concentration of 8 mmol/l, the buffering power of leucocytes was measured at different internal pH values. 3...
Articles
Clin Sci (Lond) (1989) 77 (2): 157–160.
Published: 01 August 1989
...J. Main; T. H. Thomas; R. Wilkinson 1. Previous published measurements of leucocyte cation content are inconsistent, with sodium concentrations having particularly high coefficients of variation. We have measured the effects of recovery time after isolation, different types of handling...
Articles
Clin Sci (Lond) (1988) 75 (5): 521–526.
Published: 01 November 1988
...L. L. Ng; M. Harker; E. D. Abel 1. Leucocyte Na + influx in media containing 10 mmol/l Na + was studied directly using a triple-isotope method for measuring initial 22 Na uptake rates in 20 normal and 18 untreated hypertensive subjects. The effects of 1 mmol/l amiloride (a Na + -H + -antiport...
Articles
Clin Sci (Lond) (1988) 75 (2): 179–184.
Published: 01 August 1988
...L. L. Ng; M. Harker 1. A triple-isotope method for measuring initial 22 Na uptake rates in unactivated human leucocytes in the presence of serum, using 3 H 2 O, [ 14 C]sucrose and 22 Na, is described. 2. This 22 Na influx is inhibited by amiloride, with half-maximal inhibition at a concentration...
Articles
Clin Sci (Lond) (1987) 73 (4): 387–393.
Published: 01 October 1987
...Rachel J. Green; D. N. Baron 1. Acute ethanol consumption in human volunteers did not appear to alter active cation fluxes by Na + ,K + -ATPase, or intracellular electrolyte concentrations, in peripheral leucocytes or erythrocytes. 2. Urinary electrolyte excretion was decreased after ethanol...
Articles
Clin Sci (Lond) (1987) 72 (2): 171–179.
Published: 01 February 1987
.... As erythrocytes are non-nucleated and atypical cells, we have studied electrolyte status in thyroid disease using mixed leucocytes as well; the results obtained differed from those in erythrocytes. 3. When compared with findings in healthy subjects, leucocyte Na + ,K + -ATPase activity, [ 3 H]-oubain binding...
Articles
Clin Sci (Lond) (1986) 71 (6): 737–742.
Published: 01 December 1986
...L. L. Ng; T. D. R. Hockaday 1. Human leucocyte sodium pump activity was studied in normal fasting subjects by measuring the ouabain-sensitive 22 Na + efflux rate constants. 2. This 22 Na + efflux rate constant was inversely related to the fasting plasma non-esterified fatty acid level ( r s = −0.73...
Articles
Clin Sci (Lond) (1986) 70 (6): 583–586.
Published: 01 June 1986
...H. H. Gray; P. J. Hilton; P. J. Richardson 1. This study has confirmed that serum from patients with essential hypertension inhibits sodium transport and elevates intracellular sodium in normal human leucocytes in vitro when compared with that of well matched normotensive controls. 2. The magnitude...
Articles
Clin Sci (Lond) (1986) 70 (5): 461–468.
Published: 01 May 1986
...Jonathan S. Mann; Andrew G. Renwick; Stephen T. Holgate 1. Human leucocytes whose adenine nucleotide pool was prelabelled with [ 3 H]adenine were investigated for their capacity to release adenosine and its metabolites and histamine when activated with the calcium ionophore A23187...
Articles
Clin Sci (Lond) (1985) 68 (5): 511–515.
Published: 01 May 1985
...N. A. Boon; C. Harper; J. K. Aronson; D. G. Grahame-Smith 1. We have measured intracellular sodium concentrations and specific 3 H-labelled glycoside binding characteristics in the erythrocytes and leucocytes of patients with untreated essential hypertension, and have compared the results...
Articles
Clin Sci (Lond) (1985) 68 (5): 517–521.
Published: 01 May 1985
...D. N. Baron; Rachel J. Green; F. A. Khan 1. Hypokalaemia may be produced in man by intravenous adrenaline infusion, or as a result of pathological disturbances which have led to a high plasma adrenaline concentration. 2. With isolated human leucocytes used as a cellular model, adrenaline...
Articles
Clin Sci (Lond) (1985) 68 (2): 143–149.
Published: 01 February 1985
...D. N. Baron; F. A. Khan 1. The Na + , K + -ATPase activity of human leucocytes was assayed by measuring the release of inorganic phosphate (Pi) from ATP. 2. The maximum enzyme activity was achieved under the following conditions: concentration (mmol/l), Tris/HCl 50, Na 100, K 15, ATP 5, Mg 7, EDTA...
Articles
Clin Sci (Lond) (1983) 64 (5): 505–510.
Published: 01 May 1983
...H. Schlebusch; M. Sorger; A. Höck; F. KrüCk 1. in twenty normal subjects and five patients with disturbances of potassium balance, the potassium concentration in leucocytes was measured by a modified technique. 2. The precision of the method was three to four times greater than that of techniques...
Articles
Clin Sci (Lond) (1983) 64 (3): 333–340.
Published: 01 March 1983
... Society 1983 erythrocytes exercise leucocytes metabolism plasma purines transport urine Clinical Science (1983) 64,333-340 333 Purine transport and metabolism in man: the effect of exercise on concentrations of purine bases, nucleosides and nucleotides in plasma, urine, leucocytes...
Articles
Clin Sci (Lond) (1981) 61 (s7): 5s–10s.
Published: 01 December 1981
...D. C. Tosteson; N. Adragna; I. Bize; H. Solomon; M. Canessa erythrocytes leucocytes lithium—sodium cotransport ouabain-sensitive sodium—potassium pump sodium—potassium cotransport Clinical Science (1981) 5s-10s 5s STATE OF THE ART REVIEW Membranes, ions and hypertension D . C . T O S T E...
Articles
Clin Sci (Lond) (1980) 59 (5): 353–357.
Published: 01 November 1980
...R. B. Jones; P. J. Hilton; J. Michael; J. Patrick; V. E. Johnson 1. The transport of zinc has been studied in normal human leucocytes incubated in both a tissue culture medium and Krebs buffer. 2. 65 Zn influx is characterized by an initial rapid phase followed by a slower influx. 65 Zn influx...
Articles
Clin Sci (Lond) (1980) 58 (5): 403–409.
Published: 01 May 1980
...D. C. S. Hutchison; R. Desai; D. Bellamy; H. Baum 1. The lysosomal enzymes of circulating polymorphonuclear leucocytes contain a potent elastase; release of this enzyme within the lung is thought to be responsible for the destruction of elastic tissue in pulmonary emphysema. 2. The release...
Articles
Clin Sci (Lond) (1979) 57 (4): 295–303.
Published: 01 October 1979
... occurred in mean N -acetyl-β-glucosaminidase activity in the active colitic group. 25 1 1979 14 5 1979 © 1979 The Biochemical Society and the Medical Research Society 1979 colitis leucocytes muramidase peptide hydrolases regional enteritis vitamin B 12 -binding proteins...
Articles
Clin Sci Mol Med (1978) 55 (4): 413–415.
Published: 01 October 1978
... . It is unlikely that an appreciable proportion of this oxygen is incorporated into the organic composition of the cell or of the ingested microorganism. 3 3 1978 22 6 1978 © 1978 The Biochemical Society and the Medical Research Society 1978 granulocytes hydrogen peroxide leucocytes...
Articles
Clin Sci Mol Med (1978) 54 (3): 233–240.
Published: 01 March 1978
...M. Davies; A. J. Barrett; J. Travis; E. Sanders; G. A. Coles 1. Human polymorphonuclear leucocyte elastase and cathepsin G were incubated with preparations of isolated human glomerular basement membrane at neutral pH and 37°C. 2. The ability of these enzymes to degrade glomerular basement membrane...
Articles
Clin Sci Mol Med (1977) 52 (3): 325–328.
Published: 01 March 1977
...G. E. Levin; D. N. Baron 1. Human leucocytes were isolated from venous blood and resuspended in the subject's plasma. 2. Intracellular pH measurements were made in vitro by the dimethyloxazolidinedione technique in 13 healthy subjects and in 11 subjects with renal failure and metabolic acidosis. 3...
Articles
Clin Sci Mol Med (1976) 51 (1): 87–92.
Published: 01 July 1976
... in the protective mechanisms against endogenous proteases. 5 3 1976 © 1976 The Biochemical Society and the Medical Research Society 1976 α-globulins leucocytes macroglobulins neutrophils trypsin, trypsin inhibitors Clinical Science and Molecular Medicine (1976) 51, 87-92. The disappearance...
Articles
Clin Sci Mol Med (1976) 50 (4): 293–299.
Published: 01 April 1976
...G. E. Levin; P. Collinson; D. N. Baron 1. Viable human leucocytes were isolated from venous blood and suspended in artificial media. Intracellular pH measurements were made by the dimethyloxazolidinedione technique in conditions simulating ‘respiratory’ or ‘metabolic’ acid-base disturbances. 2...
Articles
Clin Sci Mol Med (1975) 49 (6): 603–608.
Published: 01 December 1975
...J. Dow; N. Krasner; A. Goldberg 1. Hepatic alcohol dehydrogenase activity and leucocyte ascorbic acid content were measured in thirty-five patients with liver disease and in ten control subjects with duodenal ulcer. The patients with liver disease were divided into three groups consisting of non...
Articles
Clin Sci Mol Med (1975) 49 (2): 171–182.
Published: 01 August 1975
... The Biochemical Society and the Medical Research Society 1975 granulocytes leucocytes lysosomes microsomes mitochondria plasma membranes subcellular fractionation vitamin B 12 vitamin B 12 -binding proteins Clinical Science and Molecular Medicine (1975) 49, 171-1 82 Analytical subcellular...
Articles
Clin Sci Mol Med (1975) 48 (5): 441–451.
Published: 01 May 1975
... ferritin, suggesting a difference in isoelectric point. The elution point of serum ferritin from patients with acute myeloblastic leukaemia is significantly different from normal. 3. Density gradient centrifugation in sucrose showed that ferritin in leucocyte extracts and partially purified ferritin from...
Articles
Clin Sci Mol Med (1975) 48 (1): 67–73.
Published: 01 January 1975
... in both hepatectomized and normal rats and there was no difference in the proportion of pteroylpentaglutamate to other forms of folate in the regenerating or resting liver. 3. Folate concentrations were also measured in leucocytes from normal and leukaemic patients. 4. Pteroylpolyglutamates constituted...
Articles
Clin Sci Mol Med (1974) 46 (5): 613–617.
Published: 01 May 1974
...P. J. Hilton; J. Patrick 1. Sodium and potassium transport rates were studied in normal human leucocytes exposed to iso-osmolal and hypo-osmolal extracellular fluid. 2. Hypo-osmolality of the extracellular fluid led to an increase in sodium influx and a decrease in potassium influx expressed...
Articles
Clin Sci Mol Med (1973) 44 (5): 439–445.
Published: 01 May 1973
...P. J. Hilton; J. Patrick 1. Sodium and potassium efflux and influx rates were studied in normal human leucocytes in an artificial extracellular fluid using radioactive isotopes. 2. The rate constant for sodium efflux was 4.2 h −1 corresponding to a sodium efflux rate of 487 mmol kg cell dry weight...
Articles
Clin Sci (1972) 42 (4): 415–421.
Published: 01 April 1972
...J. Patrick; Berenice Bradford 1. Potassium depletion was induced in rabbits with frusemide and measurements were made of plasma, leucocyte, erythrocyte and total exchangeable potassium before and during potassium depletion. 2. All measurements, except erythrocyte potassium expressed as mmol/kg...