... a confocal microscope (LSM800, Carl Zeiss, Oberkochen, Germany). acute lung injury Forsythoside A lipopolysaccharide miR-124 STAT3 Acute lung injury (ALI) is a life-threatening disease with severe inflammatory responses. In clinic, ALI is characterized by pulmonary inflammation, increased...

... research attention. Blockade of α-2 or α2A-adrenoceptor (α 2A -AR) has been shown to attenuate lung injury induced by lipopolysaccharide (LPS) in rats. However, the mechanism is unclear. We confirmed the role of α 2A -AR in ARDS using knockout mice and alveolar macrophages following LPS stimulation...

... abundance. We predicted significantly increased abundance of the lipopolysaccharide (LPS)-synthesis pathway and significantly decreased abundance of the G protein-coupled receptor (GPCR) pathway in patients with PE, based on phylogenetic reconstruction of unobserved states (PICRUSt). Finally, we evaluated...

... signalling in mice with lipopolysaccharide (LPS)-induced lung injury. Acute LPS exposure significantly up-regulated EphA2 and EphrinA1 expression. Compared with LPS+IgG mice (IgG instillation after LPS exposure), LPS+EphA2 mAb mice [EphA2 monoclonal antibody (mAb) instillation posttreatment after LPS...

... irreversible inhibitor of P2Y 12 receptor-induced platelet activation may reduce activation of coagulation in a human LPS (lipopolysaccharide) model. A double-blind, randomized, crossover trial with a minimum washout period of 6 weeks was performed. Sixteen subjects were randomly assigned to a treatment group...

... of lipopolysaccharide (LPS) into mice mimics the immunosuppression associated with sepsis. Myeloid-derived suppressor cells (MDSCs, Gr-1 + CD11b + ) are considered a major component of the immunosuppressive network, interfering with T-cell responses in many pathological conditions. We used LPS-immunosuppressed...

... of lipopolysaccharide (LPS)-induced vascular alterations as well as studying a probable functional association of nmMLCK with nuclear factor κ-light-chain enhancer of activated B cells (NF-κB). Aortic rings from mice were exposed in vitro to LPS and, then, vascular reactivity was measured. Human aortic endothelial...

...María Gabriela Morales; Hugo Olguín; Gabriella Di Capua; Enrique Brandan; Felipe Simon; Claudio Cabello-Verrugio Skeletal muscle atrophy induced during sepsis syndrome produced by endotoxin in the form of LPS (lipopolysaccharide), is a pathological condition characterized by the loss of strength...

... of defined aerosol doses during measurement was developed. It was hypothesized that LPS (lipopolysaccharide)-challenged marmosets show AHR compared with non-challenged healthy subjects. Invasive plethysmography was performed in 12 anaesthetized orotracheally intubated and spontaneously breathing marmosets...

... pathways at the same time in order to prevent endotoxaemia is difficult. In a previous study we observed that activation of P2X 7 receptors elicited the release of IL (interleukin)-1β from LPS (lipopolysaccharide)-incubated vessels. In the present study, we hypothesize that P2X 7 receptor activation...

... and OA in non-weight-bearing joints; however, none of these hypotheses have been demonstrated empirically. In the present Hypothesis article, we recognize that obesity is associated with compromised gut mucosa, translocation of microbiota and raised serum LPS (lipopolysaccharide). The consequent...

... have demonstrated that, in patients with Type 2 diabetes categorized into low (<1.0 mg/l), medium (1.0–3.0 mg/l) and high (>3.0 mg/l) hs-CRP groups, serum levels of hs-CRP correlated with lipopolysaccharide-stimulated release of interleukin-1β and interleukin-6 in whole blood. This finding may...

... blood with LPS (lipopolysaccharide) or saline. The basal values of IL-1β, IL-6 and TNF-α were low and were not significantly related to hs-CRP levels. A univariate analysis showed that the level of IL-1β and IL-6, obtained after 24 h of incubation of whole blood with LPS, increased significantly...

... ]phenylalanine and L -[phenyl- 2 H 2 ]tyrosine to three anaesthetized experimental groups: mice receiving saline intraperitoneally (i.p.) (CON), mice receiving saline i.p. and starved for 9 h (STA), and mice receiving lipopolysaccharide i.p. and starved for 9 h (STA + LPS). The contribution of myofibrillar...

... antagonists on haemodynamics, survival rate and expression of ET-1, ET receptors and iNOS. Administration of bacterial lipopolysaccharide (LPS) into rats caused a profound hypotension with resultant death. However, these effects were blocked by a non-selective ETA/ETB receptor antagonist (TAK044...

... conditions of neonatal endotoxaemia. Suckling Wistar rats were injected intraperitoneally with 200 μ g/kg lipopolysaccharide. Oxygen consumption was measured polarographically in hepatocytes respiring on either palmitate (0.5mM) or palmitate plus glutamine (10mM). Total hepatocyte oxygen consumption...

... activated with lipopolysaccharide (LPS) at pH o 5.5, 6.5 or 7.4. TNF-α gene transcription was monitored by Northern blot analysis. Synthesis of the cytokine was monitored by ELISA measurements of the TNF-α content of cell-conditioned media (extracellularly released TNF-α) and cell lysates (cytosolic TNF-α...

... of endotoxins [lipopolysaccharide (LPS)] into the circulation and associated cytokine production. Therefore we collected blood samples from 29 athletes before, immediately after, and 1, 2 and 16 h after a long-distance triathlon for measurement of LPS, tumour necrosis factor-α and interleukin-6 (IL-6...

...Christine Fagny; Arnaud Marchant; Eric De Prez; Michel Goldman; Monique Deschodt-Lanckman 1. As lipopolysaccharide is a major stimulator of neutrophil responses during Gram-negative bacterial infections, we studied its effect on the membrane expression of neutral endopeptidase 24.11/CD10...

... was used. Adult rats were given a single sub-lethal dose of lipopolysaccharide by the intraperitoneal route. At various times thereafter, rats were killed and ventricular muscle was removed. RNA was extracted and transferred to nylon membranes. Changes in expression of mRNA for α- and β-myosin heavy chain...

... endotoxin Escherichia coli Gram-negative bacteraemia lipopolysaccharide tumour necrosis factor-α Clinical Science (1 990) 79, 6 19-623 619 Endothelin immunoreactivity in mice with Gram-negative bacteraemia: relationship to tumour necrosis factor- a KAZUHIRO TAKAHASHI, AYONA SILVA*, JONATHAN COHEN...

... immunoglobulin G. 3. Lipopolysaccharide extracted from the homologous organism was as effective as whole bacterial cells in removing the factor from patient's serum. 27 7 1972 © 1972 The Biochemical Society and the Medical Research Society 1972 serum bactericidal activity immunoglobulin G...