... group compared with 39±6fmol·mg -1 of protein for the control. There was no change in the density of the smaller population of the ET B sub-type. In agreement, the ratio of ET A mRNA to cyclophilin mRNA (a housekeeping gene) measured by Northern analysis was reduced in Ad.CMV.ET-1 rats compared...

... and rectal tissues were examined for immunohistochemistry. Altogether, 17 tissue specimens were used for enzyme assay, and eight for RNA analysis. 17βHSD2 activity was detected in the stomach, duodenum, ileum, colon and rectum. 17βHSD2 mRNA was most abundant in the small intestine. 17βHSD2 immunoreactivity...

.... Haemodynamics, lung weight gain (LWG), capillary filtration coefficient ( K fc ), tissue pathology, the concentrations of cytokines in the perfusate, and mRNAs for the various cytokines in the lung tissues were measured. I/R induced a permeability type of pulmonary oedema, as reflected by increases in LWG and K...

... of mtDNA by culture in ethidium bromide, and differential display reverse transcriptase–PCR (DDRT-PCR) was then employed to compare mRNA expression between wild-type, mtDNA-replete (ρ + ) and ethidium bromide-treated, mtDNA-depleted (ρ 0 ) cells. Expression of the majority of nuclear-encoded genes...

... in non-alcoholic than in alcoholic cirrhotic patients. By Northern blot, inducible nitric oxide synthase mRNA expression was observed only in lympho-mononuclear cells from non-alcoholic cirrhotic patients. 5. Our patients show a correlation between nitric oxide synthesis, endotoxin levels...

...Andrew T. McKie; Robert J. Simpson; Simon Ghosh; Timothy J. Peters; Farzin Farzaneh 1. In the present study we have examined the expression of pancreatitis-associated protein mRNA in mouse pancreas and small intestine and determined the effect of a number of factors on the steady-state level...

... receptor mRNA by in situ hybridization histochemistry using oligonucleotide probes to the V1a receptor and Northern blotting. 2. Diabetes mellitus caused hyperglycaemia, hyperosmolality and increased plasma vasopressin concentrations ( P < 0.01). Hepatoycte V1a receptor mRNA was reduced by 76...